高效液相色谱-三重四级杆质谱联用法检测荷斯坦和水牛原料乳中喹诺酮残留

Determination of quinolone residues in raw Holstein and Buffalo milk by high performance liquid chromatography-tandem mass spectrometry

目的建立高效液相色谱-三重四级杆质谱联用法检测我国南方地区荷斯坦和水牛原料乳中喹诺酮类抗生素类残留的分析方法。方法采集南方地区15个荷斯坦牧场和15个水牛牧场的120份原料乳,采用高效液相色谱-三重四级杆质谱联用法检测其中的喹诺酮类抗生素残留情况。结果检出限和回收率分别为0.05-0.25μg/L和88.2%-109.2%。水牛乳中喹诺酮类抗生素残留检出率为8.06%,荷斯坦牛乳中抗生素残留率为6.89%。结论该方法检测牛乳中喹诺酮类结果准确可靠,南方地区的荷斯坦牛乳和水牛乳喹诺酮类残留量与国内外相比程度较低,原料乳总体上处于安全范围。

Objective To establish a method for the determination of quinolone residues in raw Holstein and Buffalo milk in south China by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）.Methods One hundred and twenty milk samples were collected from Holstein and Buffalo farms in Zhejiang and Guangxi,and the quinolones residues in raw milk were determined by HPLC-MS/MS.Results The LODs and recovery rates were 0.05-0.25 μg/L and 88.2%-109.2%,respectively.The detection rates of quinolone residues in raw Buffalo and Holstein milk were 8.06%and 6.89%.Conclusion This method is accurate and reliable for the determination of quinolones residues in raw milk.Compared to the results of other parts and area,the quinolone residues in raw Holstein and Buffalo are in safety situation in south China.