广西HIV/AIDS患者合并感染隐孢子虫现况及基因分型初步研究

Preliminary study on infection status and gene types of Cryptosporidium among HIV/AIDS patients in Guangxi

目的了解广西艾滋病病毒感染/艾滋病(HIV/AIDS)患者隐孢子虫感染情况及虫株类型。方法收集广西南宁、桂林、钦州、百色及河池等地285名HIV/AIDS患者和南宁150名HIV阴性人群粪便样本,分别采用改良抗酸染色法和基于18S rRNA基因巢式PCR方法进行隐孢子虫检测,并对巢氏PCR产物进行测序和同源性比对,以明确基因型。结果广西HIV/AIDS患者隐孢子虫感染率为0.70%(2/285),其中伴有慢性腹泻者感染率为6.67%(2/30),HIV阴性者隐孢子虫感染率为0(0/150),后两者差异有统计学意义(P=0.002)。2例合并感染隐孢子虫的HIV/AIDS患者均来自桂林,虫株基因鉴定为安氏隐孢子虫和人隐孢子虫。结论广西HIV/AIDS患者存在隐孢子虫合并感染,感染虫株为安氏隐孢子虫和人隐孢子虫。

Objective To understand the infection status and gene types of Cryptosporidium among HIV/AIDS patients in Guangxi Zhuang Autonomous Region. Methods The fecal samples were collected from 285 HIV/AIDS cases in Nanning,Guilin,Qinzhou,Baise,Hechi cities of Guangxi and 150 HIV negative persons in Nanning City. The modified acid-fast staining and nested-PCR based on 18 S rRNA were employed to detect the infection status of Cryptosporidium. The nested PCR products were sequenced,and the homology searches and identification for the gene types of Cryptosporidium were done by DNAStar software. Results The infection rate of Cryptosporidium in HIV/AIDS patients was 0.70%（2/285）,and the rate of those with chronic diarrhea was 6.67%（2/30）,the latter was significantly higher than that of the HIV negative persons（0,0/150）（P =0.002）. Both the two HIV/AIDS patients infected with Cryptosporidium were from Guilin City. By molecular identification,the Cryptosporidium strains which the above 2 patients were infected with were Cryptosporidium andersoni and Cryptosporidium hominis respectively. Conclusions Cryptosporidium co-infection can be found in HIV/AIDS patients in Guangxi. The genotypes of the infection strains include Cryptosporidium andersoni and Cryptosporidium hominis.