基于psbA-trnH序列DNA条形码技术的绵马贯众鉴定研究

Identification of Dryopteridis Crassirhizomatis Rhizoma based on psbA-trnH barcode

利用psbA-trnH序列对绵马贯众药材、基原植物进行真伪鉴定。对实验样本的psbA-trnH序列进行聚合酶链式反应(polymerase chain reaction,PCR)扩增及产物测序,为扩大研究范围,从Gen Bank中下载了3属8种植物的psbA-trnH序列。利用DNAMAN 8.0软件进行峰图的查看、拼接及比对,使用MEGA 6.0软件计算K2P遗传距离并建立系统聚类树对其进行分析。结果显示psbA-trnH序列可对绵马贯众、其基原植物粗茎鳞毛蕨,以及同科属植物进行鉴别,其中绵马贯众与其基原植物粗茎鳞毛蕨的psbA-trnH序列完全一致,并可与其他易混药材及植物明显区分。表明psbA-trnH序列对绵马贯众药材、基原植物及其混伪品的鉴别具有可行性,为后续蕨类药材的鉴定研究提供一定的科学基础。

To identify origin of the medicinal materials Dryopteridis Crassirhizomatis Rhizoma by using the psbA-trnH sequence, the polymerase chain reaction （PCR） amplification and product sequencing of tile experimental samples were performed. In order to expand the scope of the study, the psbA-trnH sequences of 8 genera and 3 species were downloaded from GenBank for analysis. DNAMAN 8.0 software was used to show splicing and comparison results of the peak diagrams with analysis of them, and MEGA 6. 0 software was to calculate K2P genetic distances and establish clustering tree adjacent genus. The results showed that by using the psbA-trnH sequence, Dryopteridis Crassirhizomatis Rhizoma, its original plant and other easy-confused medicinal materials and plants can be distinguished with each other obviously, with the psbA-trnH sequence of Dryopteridis Crassirhizomatis Rhizoma completely consistent with that of its original plant. Consequently, it is revealed that it＇s feasible to identify Dryopteridis Crassirhizomatis Rhizoma and its original plant, and separate from its aduherants by means of the psbA-trnH sequence, which can provide more scientific bases for the further study of the identification of the ferny medicinal herbs.