维生素K2联合5-氟尿嘧啶对肝癌细胞增殖、迁移与侵袭能力的影响

Effects of Vitamin K2 Plus 5-Fluorouracil on Proliferation, Migration, and Invasiveness of Hepatocellular Carcinoma Cells

目的探讨维生素K2(VK2)联合5-氟尿嘧啶(5-FU)对肝癌细胞体外增殖、迁移和侵袭的影响。方法体外培养人肝癌PLC/RAF/5细胞,分别用VK2(100μmol/L)、5-FU(10μg/m L)及VK2与5-FU联合用药处理细胞24 h,另设置不加药的细胞作为对照组,分别采用CCK-8法、划痕试验、Matrigel侵袭小室法检测细胞增殖、迁移及侵袭情况。结果 VK2或5-FU单独作用细胞后,PLC/RAF/5细胞增殖、迁移和侵袭能力较对照组均明显降低(P<0.05);而与任一单独用药比较,VK2与5-FU联合用药对细胞增殖、迁移和侵袭能力的抑制作用均进一步明显增强(P<0.05)。结论 VK2与5-FU联合应用可能成为抑制肝癌细胞生长、迁移和侵袭的有效方法。

Objective To investigate effects of vitamin K2 in combination with 5-fluorouracil （5-FU） on proliferation, migration, and invasiveness of hepatocellular carcinoma cells in vitro. Methods Human hepatocellular carcinoma PLC/RAF/5 calls were cultured in vitro and exposed to vitamin K2 （10 μmol/L） and 5-FU （10 μg/mL） alone or in combination for 24 h. The cell proliferation, migration, and invasiveness were measured by CCK-8 assay, wound-cratch assay, and Matrigel invasion chamber assay, respectively. Results The abilities of proliferation, migration, and invasion of PLC/RAF/5 cells were significantly decreased after either alone vitamin K2 or 5-FU treatment （all P〈0.05） as compared with the control ceils, and above effects were further enhanced by the vitamin K2 in combination with 5-FU treatment as compared with either alone drug treatment （all P〈0.05）. Conclusion Combination use of vitamin K2 and 5-FU might be an effective method for inhibiting growth, migration, and invasiveness of hepatocellular carcinoma cells.