microRNA-21在不同分期胃癌中的表达及意义

Expression of microRNA-21 in Gastric Cancer of Different Tumor Stages

目的 测定micro RNA-21（mi RNA-21）在不同分期胃癌中的表达量并探讨其临床价值。方法 采用实时定量PCR技术,检测2015年1月至2016年1月期间于上海交通大学医学院附属新华医院及崇明分院普外科行手术治疗且经病理证实的50例胃癌患者的癌组织、癌旁正常胃组织和血清以及50例非胃癌患者血清中mi RNA-21的相对表达量。结果 胃癌组织中mi RNA-21的相对表达量明显高于癌旁正常胃组织（P〈0.05）,且其在TNM分期为Ⅰ期、Ⅱ期、Ⅲ期的胃癌患者癌组织中的相对表达量分别为2.17（1.48～2.90）、4.08（2.30～4.86）、8.64（5.82～18.20）,3期间的相对表达量比较差异有统计学意义（P〈0.05）。胃癌患者血清中mi RNA-21的相对表达量明显高于非胃癌患者血清中的相对表达量（P〈0.05）,其在Ⅰ期、Ⅱ期、Ⅲ期的胃癌患者血清中的相对表达量分别为31.00（24.60～37.15）、39.10（28.90～39.80）、44.15（38.95～56.68）,3期间的相对表达量比较差异有统计学意义（P〈0.05）。胃癌患者血清和胃癌组织中mi RNA-21的相对表达量呈正相关（r=0.86,P〈0.05）。结论 胃癌患者的血清和胃癌组织中mi RNA-21高表达与其TNM分期有着潜在的联系,其可能成为术前预测胃癌TNM分期的潜在指标。

Objective To quantitate expression ofmicroRNA-21 （miRNA-21） in gastric cancer of different tumor stages and discuss its clinical value. Method The relative expressions ofmiRNA-21 were quantitated in the cancer tissues, corresponding normal gastric tissues adjacent to gastric cancer, and serums of 50 gastric cancer patients received operation and confirmed gastric cancer by pathology and the serums of nongastric cancer patients in Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine and its Chongming Branch from January 2015 to January 2016 by real time quantitative PCR. Results The relative expression level of miKNA-21 in the gastric cancer tissues was significantly higher than that in the normal gastric tissues ad）acent to gastric cancer. Among the TNM stage Ⅰ, Ⅱ, Ⅲ of gastric cancer patients, the relative expression levels of miRNA-21 in the cancer tissues were 2.17 （1.48-2.90）, 4.08 （2.30-4.86）, 8.64 （5.82-18.20）, respectively and the differences among these three stages were statistically significant （P〈0.05）. The relative expression level of the serum miRNA-21 in the gastric cancer patients was significantly higher than that in the nongastric cancer patients, which in the serums for stage Ⅰ, Ⅱ, and Ⅲ patients were 31.00 （24.60-37.15）, 39.10 （28.90-39.80）, 44.15 （38.95-56.68）, respectively and the differences among three stages were statistically significant （P〈0.05）. The relative expression level of miRNA-21 in the serums and cancer tissues had a positive correlation （r=-0.86, P〈0.05）. Conclusion MiKNA-21 appears to have a potential association with TNM stage of gastric cancer, which cautiously suggests that it might be a potential indicator for prediction of preoperative TNM stage of gastric cancer.