蛇黄肝炎汤含药血清对免疫抑制大鼠免疫功能的调节作用

Effects of Shehuang Ganyan Composition medicated serum on immunologic functions in immunosupressive rats

目的:观察蛇黄肝炎汤含药血清对环磷酰胺致免疫抑制模型大鼠免疫功能的影响。方法:通过注射环磷酰胺建立免疫抑制模型大鼠,制备免疫抑制大鼠脾T淋细胞及腹腔巨噬细胞,采用MTT法检测蛇黄肝炎汤含药血清对脾T淋巴细胞增殖及腹腔巨噬细胞吞噬功能的影响。结果:不同浓度含药血清均能协同有丝分裂原ConA刺激免疫抑制大鼠脾T淋巴细胞的增殖,并可明显促进免疫抑制模型大鼠PMφ吞噬能力。结论:蛇黄肝炎汤含药血清可通过增强机体免疫功能,从而发挥抗肿瘤作用。

OBJECTIVE To investigate immunomodulation of Shehuang Ganyan Composition on immunosuppressive rats induced by cyclophosphamide（CTX）.METHODS Immunosuppressive rats were induced by CTX.Medicated serum of Shehuang Ganyan Composition was used to culture T lymphocytes and peritoneal macrophages（PMφ）in vitro.Effects of medicated serum on proliferation of splenic T lymphocytes and activities of PMφwere measured by MTT assay.RESULTS Medicated serum at different concentrations and ConA could synergistically stimulate T lymphocyte proliferation and promote PMφphagocytic function in immunosuppressive rats.CONCLUSION Medicated serum of Shehuang Ganyan Composition can improve immunological functions in immunosuppressive rats induced by CTX,thus produce antitumor effects.