壳聚糖/羟丙基甲基纤维素温敏水凝胶的制备

Preparation of Chitosan/Hydroxypropyl Methyl Cellulose Thermo-Sensitive Hydrogel

采用壳聚糖(CS)、羟丙基甲基纤维素(HPMC)为原料制备了温敏性水凝胶,分析了CS含量、HPMC含量、HPMC黏度和甘油添加量对CS/HPMC的低临界溶解温度的影响。最优条件为CS含量为1%(m/V),HPMC的黏度为6 m Pa·s,HPMC的含量为7%(m/V),甘油含量为32%(m/V),得到体系的低临界溶解温度为32℃,体系的黏度为1407 m Pa·s。红外光谱测试表明CS、HPMC与甘油之间没有化学作用,流变性测试显示体系可以在36℃下凝胶化。MTT测试结果表明CS/HPMC/Gyl无毒副作用,具有良好的生物相容性。研究结果表明该体系是一种良好的可注射型温敏水凝胶。

Temperature-sensitive hydrogels were prepared by blending chitosan（CS） with hydroxypropyl methyl cellulose （HPMC）. The effects of chitosan content, HPMC content, HPMC viscosity type and glycerol content on the lower critical solution temperature （LCST） of the formulations were analyzed. The formulation of 1% CS, 7 % HPMC （viscosity type 6 mPa·s） and 32% glycerol performs best, and LCST is brought down to 32℃ tested by ＂Visual Tube Inversion Method＂, the viscosity of the system is 1407 mPa＇s. FT-IR shows no chemical reaction among CS, HPMC and glycerol. Rheological test indicates that LCST is 36℃. MTT assay demonstrates that the CS/HPMC/Gyl system is non-toxic and biocompatible. The results show that the formulation is a good injectable thermo-sensitive hydrogel.