右冠状动脉缺血预处理对兔心肌缺血再灌注损伤的保护作用

Protective effect of right coronary artery ischemic preconditioning on myocardial ischemia reperfusion injury in rabbit heart

目的:探索右冠状动脉缺血预处理、后处理对兔的心肌缺血再灌注损伤的保护作用。方法:将30只新西兰大白兔随机分为对照组(n=7)、缺血再灌注损伤组(n=8)、右冠状动脉缺血预处理组(n=8)、右冠状动脉缺血后处理组(n=7),分别抽取术前、术后1及6 h的静脉血,测肌酸激酶同工酶、肌钙蛋白T,计算心肌梗死面积。结果:与缺血再灌注损伤组比较,右冠状动脉缺血预处理组、右冠状动脉缺血后处理组肌酸激酶同工酶、肌钙蛋白T浓度在术后1和6 h明显降低,差异均有统计学意义(均P<0.05);心肌梗死面积明显缩小,差异均有统计学意义(均P<0.05)。结论:在新西兰大白兔的心肌缺血再灌注心肌梗死模型中,右冠状动脉缺血预处理、右冠状动脉缺血后处理对心肌缺血再灌注损伤有明显的保护作用。

Objective： To explore the protective effects of right coronary artery ischemic preconditioning and post-conditioning on myocardial ischemia reperfusion injury in rabbit heart. Methods： A total of 30 rabbits were randomly divided into 4 groups： a control group （n=7）, an ischemia reperfusion group （IR group, n=8）, an ischemic preconditioning group （IPC group, n=8） and an ischemic post-conditioning group （IPO group, n=7）. Venous blood samples were taken at pre-operation, 1 and 6 h post-operation, and the concentration of serum creatine kinase isoenzyme （CKoMB） and cardiac troponin-T （cTn-T） were measured. The infarct area of cardiac muscle was calculated. Results： Compared with the IR group, the levels of CK-MB and cTn-T at 1 and 6 h post-operation in the IPC group and the IPO group were reduced （all P〈0.05）. Compared with the IR group, the infarct size in the IPC group and the IPO group was significantly decreased, with significant difference （both P〈0.05）. Conclusion： Right coronary artery ischemic preconditioning and post-conditioning exert significant protective effects on the myocardial ischemia reperfusion injury in New Zealand rabbits.