2015年郴州市布鲁氏菌病的实验室诊断及病原学特征分析

Laboratory diagnosis and etiological characteristics of brucellosis in Chenzhou City,2015

目的对2015年郴州市人感染布鲁氏菌疑似患者和病羊进行血清学和分子生物学检测,以明确诊断。方法对患者进行流行病学调查,采用血培养法对试管凝集试验检测为布鲁氏菌抗体阳性的患者和病羊血液进行荧光PCR及细菌分离培养,应用分子生物学方法和基因测序对可疑菌落进行种类鉴定。结果 68份高危及疑似布病患者血清抗体筛查13份阳性;1 679份羊血血清抗体检测57份阳性,6株分离株经荧光PCR鉴定为羊种布鲁氏菌。结论 2015年引起郴州市布病的主要病原类型为羊种布鲁氏菌,应采取综合措施防止布病在郴州流行。

Objective To clarity the diagnosis of Brucella infection in suspected patients and diseased goats in Chenzhou City in 2015 from serological and molecular biological aspects. Methods An epidemiological survey was conducted among the pa- tients. Blood culture was used to isolate and culture Brucella strains from the blood of the patients and goats with positive anti-Brucella antibody detected by the standard agglutination test, and then real-time fluorescence quantitative RT-PCR was employed for identification. The molecular biological and gene sequencing techniques were used to identify the species of the suspicious bacteria strains. Results In the serum antigen screening of 68 high-risk and suspected patients with brucellosis, 13 were detected to be positive. 57 of 1,679 serum samples from sick goats were detected to be positive, and 6 isolated strains were identified as Brucella meltensis by real-time fluorescence quantitative RT-PCR. Conclusions The main pathogen species causing brucellosis in Chenzhou City in 2015 is Brucella melitensis. Comprehensive measures should be taken to control its epidemic .