卵巢癌大网膜转移差异基因的筛选及验证

Preliminary screening and identification of differentially expressed metastasis-related genes in ovarian cancer

目的:通过筛选并验证卵巢癌原发灶和大网膜转移灶组织样本的差异表达基因,从中发现与卵巢癌大网膜转移相关的基因。方法:应用基因芯片检测3对配对的卵巢癌原发灶和大网膜转移灶组织样本差异基因表达谱,初步筛选出差异表达显著的基因,并通过qRT-PCR和免疫组化法验证基因芯片结果。结果:通过基因芯片检测共筛选得到187个差异性表达的mRNA,其中大网膜转移组相对于卵巢癌原发灶组表达上调的基因160个,表达下调的基因27个。初步筛选出差异表达显著的2个mRNA:Interleukin 8(IL-8)和Armadillo Repeat Containing 9(ARMC9);qRT-PCR和免疫组化结果显示,IL-8和ARMC9在大网膜转移灶中的表达均显著高于卵巢癌原发灶(P<0.05)。结论:IL-8和ARMC9基因可能与卵巢癌大网膜转移有关。

Objective： To find out metastasis-related genes in ovarian cancer though preliminary screenning and identificating of the diferentially expressed genes in primary ovarian cancer and omentum metastasis samples. Methods： Microarray analysis was performed to uncover genes that were differentially expressed between three pair of matched primary ovarian cancer and omental metastasis. QuantitativeReal-time PCR（ qRT-PCR） and immunohistochemistry（ IHC） were used to validate the microarray results at the mRNA and protein levels,respectively. Result： Microarray revealed that 187 mRNAs were differentially expressed. Among which160 genes were up-regulated and 27 were down-regulated in omental metastasis. Among these blotted genes,the markedly differentially expression of Interleukin 8（ IL-8） and armadillo repeat containing 9（ ARMC9） were preliminary screenned. Fourthermore,the expressions of IL-8 and ARMC9 in omentum metastasis were identified both significantly higher than that of ovarian cancer by qRT-PCR and IHC（ P〈 0. 05）. Conclusion： IL-8 and ARMC9 were related to the progress of ovarian cancer metastasize.