含甘草成分的电子烟烟油开发及表征

Preparation and characterization of e-liquids which contain licorice ingredient

为了开发新型电子烟烟油,采用超临界CO_2流体对甘草粉末进行萃取,并对萃取工艺进行了优化。最佳萃取条件为:萃取温度60℃,萃取压力400bar,动态萃取30min,静态萃取30min,夹带剂为无水乙醇,以0.2m L·min^(-1)的速度,持续加入20min。将萃取液作为电子烟烟油的基质,配制功能性的电子烟烟油,并对其进行评吸,感官评吸佳。将萃取液进行HS-SPME-GC/MS表征,结果表明:1萃取液共分离鉴别出63种化合物,其中含量较高的是烃、烯、酮、酯类化合物。2萃取液重复3次试验,组分峰面积RSD的最大值为16.8%,最小为1.0%,平均值为3.79%,重现性好。3甘草萃取液最主要的挥发性物质为2,6-二叔丁基-4-丙氧基苯酚,其次为双(三甲基硅烷基)酯-2',6'-二羟基苯乙酮、4-甲基-2-庚基-1,3-二氧戊烷、α-蒎烯、十五烷和叶绿醇。本实验为开发具有功效特色型电子烟烟液,为电子烟烟液调香技术人员提供技术依据和参考价值。

In order to develop new style e-liquids,the licorice root were extracted by supercritical CO2 fluid extraction,and the extraction process was optimized.The optimal conditions were as follows： extraction temperature： 60℃,pressure： 400 bar,dynamic extraction： 30 min,static extraction： 30 min,entrainer： absolute ethyl alcohol,entrainer flow rate： 0. 2m L·min^-1,injection time： 20 min.The new style functional e-liquids were confected by the extract liquor.The smoking experiment showed that the extract liquor could reduce irritation and improve smoking taste effectively.The volatile chemical constituents of the extract liquor was separated and identified by headspace solid-phase microextraction（ HS-SPME）,followed by gas chromatography-mass spectrometry（ GC-MS）. The results indicated that（ 1） 63 main volatile components in the e-liquids were analyzed and the main components were hydrocarbons,alkenes,ketones and esters.（2） The maximum relative standard deviations（ RSDs） of peak area was 16. 8%,the minimum was 1. 0%and the average was 3. 79%at three times experiment.（3） The most important volatile component was 2,6-Bis（ 1,1-dimethylethyl）-4-（ 1-oxopropyl） phenol and the other flavors were 2＇,6＇-Dihydroxyacetophenone,bis（ trimethylsilyl） ether,2-heptyl-4-methyl-1,3-Dioxolane,α-copaene,pentadecane and phytol.It aims providing a referenced method and technical basis to the study about preparation and characterization of new style functional e-liquids.