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2-TeCD对UVB致NIH3T3细胞损伤的保护作用

Protective Effect of 2-TeCD on UVB-Induced Damage in NIH3T3 Cells
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摘要 采用4,6-二脒基二苯基吲哚(DAPI)荧光染色法和流式细胞术考察谷胱甘肽过氧化物酶(GPx)活力的人工模拟物2-TeCD对UVB致NIH3T3细胞损伤的保护作用.结果表明:2-TeCD可影响UVB致NIH3T3细胞中DNA裂解率、脂质过氧化水平、活性氧体积分数、细胞周期及P53蛋白表达量等指标,可通过清除自由基发挥对UVB损伤的防护作用. Using fluorescence staining of 4,6-diamidino-2:phenylindole dihyodrochloride and flow cytometry we studied protective effect of an artificial mimic 2-tellurium bridged cyclodextrin (2-TeCD) with the activity of glutathione peroxidase (GPx) on uVB-induced damage in NIH3T3 cells. The results show that 2-TeCD can affect DNA lysis rate, the level of lipid peroxidation, the volume fraction of ROS, cell cycle, P53 protein expression and other indicators in NIH3T3 cells caused by UVB. The 2-TeCD can play a protective role against UVB damage by removing free radicals.
出处 《吉林大学学报(理学版)》 CAS CSCD 北大核心 2016年第6期1451-1455,共5页 Journal of Jilin University:Science Edition
基金 吉林省自然科学基金(批准号:20130101159JC) 吉林省科技发展计划项目(批准号:2015YMLS002NY)
关键词 谷胱甘肽过氧化物酶 模拟物 2-TeCD UVB gluathione peroxidase (GPx) mimic 2-TeCD UVB
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