锥栗种质资源遗传多样性的SRAP分析

The Analysis of Genetic Diversity of Castanea henryi(Skan)Rehder & E. H. Wilson by Using SRAP

应用SRAP分子标记法,采用筛选出的15个引物组合,对采自湖南、浙江、四川、贵州、福建5省的17个野生锥栗居群资源及23份锥栗栽培品种进行了遗传多样性分析,旨在为锥栗遗传背景分析和种质创新提供依据。17个野生锥栗居群共扩增出221个位点,平均多态性位点数为155.06,多态性位点百分率为70.16%。总遗传多样性指数（Ht）、居群内遗传多样性指数（Hs）、Nei＇s基因多样性指数、Shannon＇s信息指数分别为0.3636、0.2466、0.2460、0.3677,说明野生锥栗居群具有较高的遗传多样性和变异度。其中,衡山（HS）居群的多态性位点百分率最高,为85.07%,吉首小溪村（JS）居群次之,为83.26%,表明衡山、湘西一带为湖南省野生锥栗居群遗传多样性最丰富的区域。UPGMA聚类分析表明,浏阳溪江居群和衡山居群间的遗传相似性最大,亲缘关系最近;贵州黎平居群和岳阳月田镇居群间的遗传相似性最小,遗传差异较大。23个锥栗品种多态性位点百分率占比为89.14%,遗传相似性系数在0.66-0.85之间,且在遗传相似性系数0.66处,大尖嘴单独聚为一类,反映出它的遗传基础与其他品种具有较大的差异;在遗传相似性系数0.85处,华栗2号和铁锥被聚为一类,表明这2个品种亲缘关系最近,遗传差异最小。此外,还构建了Em1-Me2、Em2-Me1、Em2-Me2和Em2-Me3共4个引物组合的SRAP数字指纹图谱,为锥栗品种的快速鉴定提供了参考依据。

Applied with the SRAP molecular labeling method and 15 selected primer combinations,this paper analyzes 23 cultivars of Castanea henryi（Skan） Rehder E. H. Wilson and 17 populations resources of wild Castanea henryi（Skan） Rehder E. H. Wilson in 5 provinces（Hunan,Zhejiang,Sichuan,Guizhou and Fujian）,aming to provide a basis for genetic background analysis and germplasm innovation of Castanea henryi（Skan） Rehder E. H. Wilson. A total of 221 sites were amplified in 17 wild Castanea henryi（Skan） Rehder E. H. Wilson populations,with the average number and ratio of polymorphic loci being 155. 06 and 70. 16%. The number of total genetic diversity index（Ht）,intra-population genetic diversity index（Hs）,Nei＇s genetic diversity index and Shannon＇s information index were respectively 0. 3636,0. 2466,0. 2460 and 0. 3677,indicating relatively high genetic diversity and variability of wild Castanea henryi（Skan） Rehder E. H. Wilson populations. Among them,the polymorphic rate of Hengshan（HS）population was the highest,which accounted for 85. 07%,followed by Jishou（JS） Brook Village with 83. 26%. This evidence suggestted that populations in Hengshan and Jishou had the richest genetic diversity in Hunan province. UPGMA cluster analysis showed that Xijiang population in Liuyang and Hengshan population had the closest genetic similarity and relationship,while the contrary was the case of Liping population in Guizhou and Yuetianzhen population inYueyang. The polymorphic rate of 23 varieties of Castanea henryi was 89. 14% and the genetic similarity coefficient was between 0. 66 and 0. 85. At coefficient 0. 66,Dajianzui alone were clustered into one category,which reflected there was a big difference in genetic basis between Dajianzui and others. At coefficient 0. 85,Huali2 and Tiezhui were clustered into one category,implying they shared the closest relationship with minimum genetic diversity. In addition,during the research,we had built a SRAP digital fingerprint for the 23 varieties of Castanea henryi（Skan） Rehder E. H. Wilson by using 4 primer combinations,named Em1-Me2,Em2-Me1,Em2-Me2 and Em2-Me3,thus providing references for rapid identification of Castanea henryi（Skan） Rehder E. H. Wilson varieties.