卫气月生理变化周期中大鼠血浆代谢标志物的特征

Study on Plasma Metabolic Biomarkers of Wei Qi (卫气) Monthly Physiological Changes in Rats

目的探讨卫气月生理变化的潜在代谢标志物。方法以秋分日所在的农历月确定月初(农历八月初一)、月中(八月十五)及月末(八月三十)。将10只SD大鼠分别在相应日期的正午12:00眼眶后静脉丛采血,采用高效液相色谱-质谱联用仪检测血浆代谢物,利用主成分分析和偏最小二乘法对数据进行统计分析,比较各组之间血浆代谢组学数据,探索各组之间差异性代谢标志物,推测卫气月生理变化的潜在标志物。结果与月中比较,月初有7种差异代谢物为区分两组的可能标志物,月末有8种差异代谢物为区分两组的可能标志物;与月末比较,月初有9种差异代谢物为区别两组的可能标志物。推测其中的二十碳五烯酸、皮质醇、脯氨酸和富马酸二甲酯4种差异代谢物为卫气月生理变化的潜在差异性标志物。结论卫气的月生理变化可能与脂质代谢、氨基酸代谢密切相关,并可能受到激素水平变化的影响。

Objective To explore the potential metabolic biomarkers of Wei Qi monthly physiological changes.Methods The beginning of the month（ 1st Aug. of the lunar calendar）,the middle of the month（ 15 th Aug.） and the end of the month（ 30 th Aug.） were set by the lunar calendar month with autumnal equinox. Ten SD rats were sampled from posterior orbital venous plexus at midday 12： 00 on corresponding days. Plasma metabolites were detected by high performance liquid chromatography / mass spectrometry instrument. Data was analyzed by principal component analysis and partial least square method. Plasma metabonomics data in each group were compared. Differential metabolic markers in each group were explored. The potential metabolic biomarkers of Wei Qi monthly physiological changes were inferred. Results Compared with the middle of the month,7 differential metabolites on the beginning of the month might be possible markers to distinguish between the two groups,while 8 differential metabolites on the end of the month might be possible markers to distinguish between the two groups. Compared with the end of the month,9 differential metabolites on the beginning of the month might be possible markers to distinguish between the two groups. Four differential metabolites（ including eicosapentaenoic acid,cortisol,proline and dimethyl fumarate）were inferred as the potential metabolic biomarkers of Wei Qi monthly physiological change. Conclusion Wei Qi monthly physiological change might relate to lipid metabolism and amino acid metabolism closely and it might be influenced by change of hormone level.