改良盲肠结扎穿孔术模型对生存率的影响

Reformed method affect the survival rate of cecal ligation plus puncture model

目的 对原有盲肠结扎穿孔术（CLP）模型进行改良,使其操作更简便,重复性和一致性更好.方法 用小鼠盲肠分支血管位置作为盲肠结扎位置的标准,将CLP模型分为轻、中、重3组,对比观察不同严重程度组别小鼠生存率.增加液体复苏和广谱抗生素处理因素,观察对重度CLP模型生存率影响.结果 对照组和轻度组72 h生存率为100％,中度组72 h生存率为70％,重度组40h生存率为0.复苏组和抗生素组的全部死亡时间分别为44h和46h,与重度CLP组比较差异无统计学意义（P＜0.05）.结论 该方法改良CLP模型,可获得不同严重程度表现,操作简便,可重复性和一致性好.液体复苏和广谱抗生素不能明显改变重度CLP模型小鼠生存率.

Objective To make cecal ligation plus puncture model more convenient and get better repeatability and consistency by reforming.Methods We observer the different survival rate in three groups of mild,moderate and severe degree.The standard is based on the positions of arterial branches on the cecum.We also observe the changes of survival rate when administrating fluid resuscitation and widespectrum antibiotics.Results The survival rate of mild group and control are 100％ at 72 hours,moderate is 70％.The survival rate of severe group is 0 at 40 hours.All mice died in resuscitation and antibiotics group at 44 and 46 hours,that has no significant difference with severe group.Conclusion Different CLP＆#39;s performances can be gotten by our reforming method.This method is handy to complete and has better repeatability and consistency.The survival rate of severe CLP model can＆#39;t be improved by fluid resuscitation and wide-spectrum antibiotics.