PEBP4基因对非小细胞肺癌细胞体外生物学行为的影响

Effects of PEBP4gene on biological behaviors of non-small cell lung cancer cells in vitro

目的探讨磷脂酰乙醇胺结合蛋白4(PEBP4)基因对于非小细胞肺癌(NSCLC)细胞体外生物学行为的影响。方法体外培养NSCLC细胞系HCC827细胞,并分为A组(PEBP4shRNA转染)、B组(无关序列转染)和C组(不做任何处理)。采用Western blot法检测PEBP4、周期蛋白D1、p53、Bcl-2、MMP-2和MMP-9蛋白的表达,MTT法、流式细胞仪和Transwell实验分析PEBP4对NSCLC细胞生物学行为的影响。结果PEBP4基因在NSCLC细胞系中呈高表达。与B、C组比较,A组细胞的活力及迁移能力降低,细胞凋亡增加,周期蛋白D1、Bcl-2、MMP-2及MMP-9蛋白表达水平降低,p53蛋白表达增加(P<0.05)。结论 PEBP4基因可促进HCC827细胞活力和迁移能力,抑制细胞凋亡。

Objective To investigate the effects of phosphatidylethanolamine-binding protein 4 （PEBP4） gene on biological behaviors of non-small cell lung cancer（NSCLC） cells in vitro. Methods The human NSCLC cell line HCC827 was cultured in vitro and divided into 3 groups of A（PEBP4 shRNA transfection）, B（negative control） and C （blank control）. Western blot was performed to examine protein expressions of PEBP4, cyclin D1, p53, Bcl-2, MMP-2 and MMP-9. MTT assay, flow cytometry analysis and transwell assay were used to messure the effect of PEBP4 on the biological behaviors of NSCLC cells. Results PEBP4 was highly expressed in the lung cancer cell lines. Compared to groups of B and C, HCC827 cells in group A showed lower viability, migration ability, and higher apoptosis. The ptotein levels of cyclin D1,Bcl-2,MMP-2 and MMP9 were lower and p53 was higher in group A than those in groups of B and C（P〈0.05）. Conclusion PEBP4 can enhance NSCLC cell viability and migration ability, inhibit cell apoptosis. The down-regulation of PEBP4 gene expression may play a role in reducing the invasion ability and increasing apoptosis of human NSCLC cell line HCC827.