HPLC法测定利心丸中毛蕊花糖苷的含量

Content Determination of Verbascoside in Lixin Pill by HPLC

目的：建立测定利心丸中毛蕊花糖苷含量的方法。方法：采用高效液相色谱法。色谱柱为ZORBAX SB-Aq,流动相为乙腈-0.1%磷酸溶液（16∶84,V/V）,流速为1.0 ml/min,检测波长为334 nm,柱温为30℃,进样量为10μl。结果：毛蕊花糖苷检测进样量线性范围为0.254 4-1.526 4μg（r=0.999 7）;定量限为1.358 6μg/ml,检测限为0.407 6μg/ml;精密度、稳定性、重复性试验的RSD〈2%;加样回收率为95.06%-97.31%（RSD=1.05%,n=6）。结论：该方法操作简便、结果准确,适用于测定利心丸中毛蕊花糖苷的含量。

OBJECTIVE ： To establish a method for the content determination of verbascoside in Lixin pill. METHODS： HPLC was performed on the column of ZORBAX SB-Aq with mobile phase of acetonitrile-1% phosphoric acid solution （16：84, V/V） at a flow rate of 1.0 ml/min, detection wavelength was 334 nm, column temperature was 30℃, and injection volume was 10 μl. RESULTS： The linear range of verbascoside was 0.254 4-1.526 4 μg（r=0.999 7）; limit of quantification was 1.358 6 pg/ml, and limit of detection was 0.407 6 μg/ml; RSDs of precision, stability and reproducibility tests were lower than 2% ; recovery was 95.06%-97.31% （RSD= 1.05% ,n=6）. CONCLUSIONS： The method is simple, accurate, and suitable for the content determina- tion of verbascoside in Lixin pill.