生物电阻抗技术评价大花月季的耐热性及抗寒性

Evaluation of Heat Resistance and Cold Resistance of Grandiflora Roses by Bioelectrical Impedance Technology

以2年生大花月季品种"黑魔术""金奖章""全美小姐"当年生枝条为试材,采用电阻抗图谱(EIS)法研究了茎在4—8月的耐热性与9月至翌年1月的抗寒性,并对高温、低温胁迫下电阻抗参数的变化进行了详细研究,以期确定各电阻抗参数对温度胁迫的响应,并得到不经温度处理估测半致死温度的最适参数及响应回归方程。结果表明:胞外电阻率re和弛豫时间τ随高温、re随低温处理的变化曲线能用Logistic方程得到较好拟合结果;不经温度处理样本最适估测高温半致死温度的参数分别为"黑魔术"胞内电阻率ri(r=0.795)、"金奖章"ri(r=0.809)、"全美小姐"τ(r=-0.858);最适估测低温半致死温度的参数分别为"黑魔术"弛豫时间发布系数ψ(r=0.967)、"金奖章"re(r=-0.873)、"全美小姐"ψ(r=0.922)。

The one-year-old shoots in two-year-old grandiflora roses ＇Magia Nera＇ Gold Medal＇ Maria Callas＇ were used as materials. The heat resistance of stems from April to August and the cold resistance of stems from September to the next January were measured by the method of electrical impedance spectroscopy （E IS）. And the changes of EIS parameters in high temperature and low temperature stresses were studied in detail. The aims of the present study were to determine the response of EIS parameters to temperature stress,and to obtain the best EIS parameters for estimating the semi-lethal temperatures without temperature test. The results showed that under high temperature stress test, the changes of extracellular resistance ro and relaxation time τ could be well fitted by Logistic equation; and under low temperature stress test, re could be well fitted by Logistic equation. For the samples without temperature test, the best EIS parameters to estimate the semi-lethal of high temperature stress were intracellular resistance ri（r=0. 795）,ri （r=0. 809） and τ（r=-0. 858） for ＇Magia Nera＂Gold Medal＇ and ＇Maria Callas＇ respectively. The best EIS parameters to estimate the semi-lethal of low temperature stress were relaxation time distribution coefficient of ψ（r= 0. 967）, re （r=-0. 873） and ψ（r=0. 922） for ＇Magia Nera＇ ＇Gold Medal＇ and ＇Maria Callas＇ respectively.