有髯鸢尾杂交种成熟胚培养成苗技术

Regeneration System for Mature Embryo of Beared Irises Hybrid Seeds

以有髯鸢尾"白与蓝"×"印度首领"杂交种子成熟胚为试材,采用组织培养法,研究了消毒方式、生长调节剂的种类与浓度对有髯鸢尾种胚成苗影响,以期为鸢尾育种提供理论依据。结果表明:成熟胚诱导最适培养基为MS+2.0mg·L^(-1) 6-BA+0.5mg·L^(-1) IBA,萌发率达86.6%,成苗率达85.0%;丛生芽在MS+4.0mg·L^(-1) 6-BA+0.5mg·L^(-1) IBA的培养基上增殖系数最大,为5.76;适宜的生根培养基为MS+0.7mg·L^(-1) PP333,接种后7d开始生根,生根率达83.33%;组培苗生根后无需练苗便可移栽至蛭石中,移栽成活率89.53%。

Mature embryo of beared Irises （geranica cv. ＇Baiyulan＇ × ger manica-x＇Indian chief＇） hybrid seeds were selected as materials. By tissue culture,the effect of disinfection methods, different types and concentrations of growth hormones on the development of seed embryo and plantlet formation was studied. The results showed that the optimal culture medium for mature embryogenesis was MS＋2. 0 mg·L-1 6-BA＋0. 5 mg·L-1 IBA, the rate of embryo germination and seedling formation could reach 86.6 % and 85.0%. During the proliferating period of clustered shoots, the medium of MS＋4. 0 mg·L-1 6-BA＋0. 5 mg·L-1 IBA showed the best effect. The multiplication coefficient was 5.76. MS＋0. 7 mg·L-1 PP333 was the optimum for rooting. The majority of root after seven days started to grow,and the rooting rate was 83.33 %. Vermiculite was the best transplanting medium for rooting plantlets. The transplant survival percent was 89. 53% without hardening-seedling.