酿酒葡萄“赤霞珠”组织培养快繁技术优化

Optimization of Tissue Culture and Rapid Propagation of ‘Cabernet Sauvignon' Grape

以酿酒葡萄"赤霞珠"为试材,采用组织培养法,研究了不同基本培养基与不同IBA浓度的组合及不同培养容器对酿酒葡萄"赤霞珠"试管苗生长的影响,以期优化酿酒葡萄"赤霞珠"试管苗培养技术体系。结果表明:适合"赤霞珠"试管苗组织培养的最佳培养基为1/2B5+0.2mg·L^(-1) IBA+30g·L^(-1)蔗糖+7g·L^(-1)琼脂+1g·L^(-1)活性炭;在透气膜直径为3.0cm,透光率为90%的玻璃培养容器中,"赤霞珠"试管苗生长健壮,移栽成活率最高,达96.67%。

Wine grape ＇Cabernet Sauvignon＇ was used as test material, tissue culture method was used, the effect of different basic culture medium with different concentration of IBA combination and different culture containers on ＇Cabernet Sauvignon＇ grape growth was studied,in order to enhance the growth potential of ＇Cabernet Sauvignon＇ grape in vitro and improve the survival rate of transplanting. The results showed that the 1/2B5 containing 0. 2 mg·L-1 IBA＋ 30 g·L-1 suerose＋7 g·L-1 agar＋1 g ·L-1 activated carbon was suitable for ＇Cabernet Sauvignon＇ grape in vitro culture. ＇Cabernet Sauvignon＇ grape seedlings grew strongly in the bottle with breathable film diameter of 3. 0 Cm and light transmission rate 90%. The transplanting survival rate was the highest 96. 67%.