一株线虫寄生菌的鉴定及其在不同农产品培养基上的培养效果比较

Identification of Nematophagous Fungus and Its Culture Efficiency on Different Agricultural Product Media

以一株粉红粘帚霉（Clonostachys rosea）CAYU-3菌株为试材,采用平板培养的评价方法,研究了该菌株在6种农产品（大豆、大米、玉米、小麦、马铃薯、花生）培养基（去渣或留渣）以及PDA培养基上的菌落生长和产孢情况。结果表明：该菌株在不同农产品培养基上的菌丝生长和菌落形态存在较大的差异。无论培养基是否留渣,在大豆、花生培养基上,该菌株的菌丝生长紧密,菌丝量多,而在玉米、大米、小麦培养基中菌丝疏松,菌丝量少,整体生长状况较差。在产孢方面,该菌株在不同的农产品培养基上产孢量存在显著差异。去渣处理对该菌株产孢量有显著影响。在去渣培养基中,花生培养基的产孢量最高（1.79×107个孢子·cm-2）;在留渣培养基中,大豆培养基的产孢量最高（2.87×107个孢子·cm-2）。由此看来,大豆和花生培养基较适合该菌株的产孢。试验结果还表明,大米培养基对该菌株的产孢不是很有利,无论去渣与否,大米培养基的产孢量均较低（0.24×107~0.32×107个孢子·cm-2）。

A nematophagous fungus isolate CAYU-3 was used as tested material, by using the plate culture method, the mycelia growth and conidia yield of this fungus were investigated on six different agricultural product media （soybean, rice,maize,wheat,potato and peanut） with or without filter. PDA medium was used as the control. The results showed that this fungus could grow and sporulate on all tested agricultural media no matter filter or without filter. The mycelia growth was significantly different on both peanut and maize media between filter and without filter. The conidia yield was also significantly affected by the filter treatment. It was the highest on peanut media （1. 79 × 107 spore· cm-2） among the filtered media,and the highest on soybean （2. 87×107 spore·cm-2） among the without filter media. The conidia yield was always the lowest on rice media both filtered and without filter. These results indicated that soybean and peanut media were the best for fungal sporulation,while rice media was the worst （0. 24 × 107 -0. 32 ×107 spore·cm-2 ）.