两株野生紫色香蘑菌株的鉴定

Identification of Two Wild Lepista Strains

以采自黑龙江省的2株野生紫色食用菌B5和BF8菌株为试材,采用形态学和分子标记方法,研究了不同香蘑的遗传多样性,以期为香蘑在分子水平鉴定和品种亲缘关系的分析提供依据。结果表明:B5和BF8子实体、菌丝的形态特征存在明显差异;2株菌株的28SrDNA高保守区序列和ITS序列的同源性分别为97.93%和89.84%。B5菌株鉴定为花脸香蘑(Lepista sordida);BF8鉴定为紫丁香蘑(Lepista nuda),它们为同属不同种的2种食用菌。

In order to provide an evidence at the molecular level about resources identification and genetic relationship analysis of Lepista mushroom. The study which used the method of the morphological features of culture and molecular markers were made to analyze the genetic diversity of different Lepista mushroom. The 2 Lepista mushrooms of B5 and BF8 collected from the Heilongjiang were used as experimental materials. The results showed that 135 and BF8 were significantly different in the fruiting bodies and mycelial characteristics. The homology of 2 the strains of 28S rDNA in highly conserved sequence and of ITS in moderately conserved region was 97.93% and 89.84%, respectively. In conclusion,B5 was Lepista sordida and BF8 was Lepista nuda ,which belonged to different species in the same genera.