TRAF6与NF-κB在特发性炎症性肌病小鼠肌肉中的表达及意义

The Expression and Significance of TRAF6 and NF-κB in the Muscle Tissue of Idiopathic Inflammatory Myopathies in Mouse

目的:研究肿瘤坏死因子受体相关因子6(TRAF6)与核转录因子κB(NF-κB)在特发性炎症性肌病(IIMs)中的表达情况,探讨TRAF6在IIMs发病中的作用及机制。方法:30只雌性BALB/c小鼠随机分为5组(每组6只),A:正常对照组;B^E:IIMs模型自第一次免疫后分别在1周、2周、3周、4周末处理组;采用实时荧光定量PCR方法检测各组小鼠肌肉组织中TRAF6与NF-κB m RNA表达水平。结果:(1)IIMs各组小鼠肌肉中TRAF6与NF-κB m RNA与正常对照组相比表达均有不同程度升高(P<0.01),第2周末时升高最为显著(P<0.01),第3周、4周呈下降趋势(P<0.01);(2)IIMs小鼠各组肌肉组织中TRAF6与NF-κB m RNA表达水平与肌肉炎症程度呈正相关(r=0.940,r=0.908,P<0.01),前二者之间也呈显著正相关(r=0.944,P<0.01)。结论:TRAF6、NF-κB m RNA表达在IIMs小鼠肌肉中上调,TRAF6可能通过NF-κB的激活在IIMs发生发展过程中发挥重要作用。

Objective： To investigate tumor necrosis factor receptor associated factor 6（TRAF6） and nuclear factor kappa B（NF-κB） m RNA levels in mouse muscle tissue of idiopathic inflammatory myopathies（IIMs） in order to understand whether TRAF6 plays a role in IIM. Methods： Thirty female BALB/c mice were randomized for five groups（6 mice in each group）： A, normal control mice; B^E, mice of IIMs model which were sacrificed at the first weekend, the second weekend, the third weekend and the fourth weekend since they had been given the first immunization for preparing IIMs mouse model. TRAF6 and NF-κB m RNA were detected with real time fluorescent PCR. Results：（1）Compared with normal control group, the expression of TRAF6 and NF-κB m RNA in each group of IIMs model had statistically significant differences（P〈0.01）and group C had the most（P〈0.01）.（2）The expression of both TRAF6 m RNA and NF-κB m RNA had positive correlations with the grading of inflammation respectively（r=0.940, r=0.908, P〈0.01）. The former two also had significantly positive correlations（r=0.944, P〈0.01）. Conclusion： TRAF6 and NF-κBm RNA expressions were upregulated in the musle tissue of IIMs, which might play an important role in the development of IIMs by activating NF-κB.