血液样本处理方式对Wistar大鼠血液生化指标检测结果的影响

Effects of different sample process modes on blood biochemical indicator detection in Wistar rats

目的观察血液样本处理方式对大鼠血液生化指标检测结果的影响。方法取体重180~220 g的SPF级Wistar大鼠40只,雌雄各半,禁食不禁水12 h后,用3%戊巴比妥钠麻醉后取全血和肝素抗凝血各3 m L。将每种血样分别置于3个1.5 m L的离心管和肝素抗凝管中各1 m L,并分别在室温放置1 h、2 h、4 h后进行离心,测定血糖、血脂、蛋白、肝功能、肾功能、心肌酶等指标。结果血液样本类型和采集后离心前的放置时间对Wistar大鼠的血清或血浆TC、TG、TP、ALB、ALT、ALP、BUN和CREA等指标均无明显影响（P〉0.05）。但血浆的GLU值显著高于血清（P〈0.05,P〈0.01）,并随着采集后离心前放置时间的延长持续下降。血清AST和CK、LDH值则均显著高于血浆（P〈0.05,P〈0.01）,且随采集后离心前放置的延长呈现持续显著的上升（P〈0.05,P〈0.01）。而采集后离心前放置时间对Wistar大鼠血浆AST和CK、LDH值均无明显影响（P〉0.05）。结论血液样本类型对血脂、蛋白和肾功能指标无明显影响,但对GLU、AST、CK、LDH等检测结果有一定影响。采集后离心前的放置时间延长可导致血清血浆GLU下降,血清AST、CK、LDH升高,但对血浆AST、CK、LDH指标无明显影响。

Objective To observe the effects of different sample process modes on blood biochemical indicator detection in Wistar rats. Methods 40 Wistar rats of specific pathogen free（ SPF） level,weighing 180 ~ 220 g,were took with half males and half females. After 12 h fasting,rats were anaesthetized by 3% pentobarbital sodium,blood was collected and divided into two samples. One of the samples was for whole blood and another for anticoagulation,with 3m L per serving. Each blood sample was put into three 1. 5 m L centrifuge tubes and three heparin anticoagulative tubes with1 m L per,and centrifuged after putting 1 h,2 h and 4 h in room temperature for testing blood glucose,blood lipid,protein,myocardial enzymes,indexes of liver and renal function. Results The types of blood samples and the standing time after collection and before centrifugation had no significant effect on TC,TG,ALB,ALT,ALP,BUN and CREA in serum or in plasma of Wistar rats（ P 0. 05）. But the value of GLU in serum was significantly higher than that in plasma（ P 0. 05,P 0. 01）. With the extension of storage time after collection and before centrifugation,the value of GLU continued to decline. The values of AST,CK,LDH in serum were significantly higher than that in plasma（ P 0. 05,P 0. 01）. With the extension of storage time after collection and before centrifugation,the value of AST,CK,LDH continued to increase.However,there was no significant effect on the values of AST,CK,LDH by placing different time after collection and before centrifugation（ P 0. 05）. Conclusion Types of blood samples had no significant effect on blood lipid,protein and indexes of renal function. However,detection value of GLU,AST,CK and LDH would be influenced on a certain degree.The extension of standing time after collection and before centrifugation resulted in plasma GLU decreasing and plasma AST,CK and LDH increasing,but has no obvious effect on the serum AST,CK and LDH.