核壳型单磷酸腺苷分子印迹聚合物的制备

Preparation of Core-Shell Adenosine-5′-Monophosphate Molecularly Imprinted Polymer

以聚苯乙烯微球(CP)为内核、单磷酸腺苷(AMP)为模板、丙烯酰胺(AM)为功能单体、乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,制备了核壳型单磷酸腺苷分子印迹聚合物(AMP-MIP),讨论了不同洗脱方式、洗脱溶剂和聚合时间对AMP-MIP吸附性能的影响,通过扫描电镜、水中悬浮实验和吸附实验对AMP-MIP的形貌和性能进行了表征,得到最佳工艺条件:洗脱方式为超声、洗脱溶剂为甲醇-乙酸(4∶1,体积比)、聚合时间为30min。分子印迹聚合物不仅对小分子模板有吸附作用,而且对大分子ADP-核糖基化的甘油醛-3-磷酸脱氢酶(gapA)具有识别作用,吸附量可达3.9μg·mg^(-1)。

Using polystyrene microsphere （CP） as a core, adenosine-5＇-monophosphate（AMP） as a template, aerylamide（AM） as a functional monomer and ethylene glycol dimethaerylate（EGDMA） as a eross-linker,while dimethylsulfoxide（DMSO） was chosen as a porogen and azobisisobutyronitrile（AIBN） as an initiator,core-shell adenosine-5＇-monophosphate molecular imprinted polymer（AMP-MIP） was prepared. The effects of different e- lution methods,solvents and polymerization time on the properties of AMP-MIP reeognition were discussed. The morphology and property of AMP-MIP were characterized by SEM,the suspension in aqueous medium and the binding experiments. The optimal process conditions were obtained as follows：the elution method was ultrasound,the elution solvent was methanol-acetic acid （4： 1） and polymerization time was 30 min. AMP-MIP showed good selectivities not only for AMP but also for glyceraldehyde-3-phosphate dehydrogenase （gapA） which was ADP-ribosylated protein. The adsorption capacity of gapA could reach 3.9μg· mg^-1.