多样本人腮腺间充质干细胞诱导iPS细胞的对比研究

Comparative study on the induction of iPS cells by multi-sample mesenchymal stem cells from human parotid gland

目的研究比较不同样本腮腺间充质干细胞(hPMSCs)生成诱导多潜能干细胞(iPSCs)的效果。方法分别分离培养3个患者的h PMSCs,应用游离质粒混合因子(OCT3/4、SOX2、KLF4、c-MYC、LIN28、TP53 shRNA)电转导法重编程诱导获得iPS细胞(h PMSC-iPSCs)。比较在相同条件下3个不同供体来源腮腺细胞诱导iPS细胞的差异。结果 3个不同样本人腮腺间充质干细胞都成功诱导iPSCs,形成率分别为0.11%、0.10%、0.09%,无显著性差异(P>0.05);诱导形成iPS克隆时间有差异(P<0.05);形成iPSCs后维持培养倍增时间无差异(P>0.05)。结论不同患者可分离获得hPMSCs后成功诱导hPMSC-iPSCs,诱导效率无差异,诱导时间有差异。

Objective To study and compare the effects of the generation of induced pluripotent stem cells（ iPSCs） from different samples of parotid mesenchymal stem cells（ hPMSCs）. Methods The h PMSCs of three patients were isolated and cultured,then were used by a combination of sufcient episomal reprogramming vectors that consist of OCT3 /4,SOX2,KLF4,L-MYC,LIN28 and shRNA targeting TP53 to generate iPSCs. The difference of iPS cells induced among three sources of parotid cells on the same condition was compared. Results Three samples of parotid gland mesenchymal stem cells were successful in inducing iPSCs,and their formation rates were respectively 0. 11%,0. 10% and 0. 09%,no significant difference（ P〉0. 05）; formation time of iPSC clones induced was different（ P〉0. 05）; the doubling time of h PMSC-iPSCs was no difference in maintaining culture after iPSCs formation（ P〈0. 05）.Conclusion The hPMSC-iPSCs can be successfully induced by hPMSCs from different patients,and there is no difference in induction efficiency,while exits the difference in induction time.