隔药饼灸对子宫内膜异位症大鼠p38MAPK信号通路的干预作用

Intervention Effect of Herbal Cake Moxibustion on p38MAPK Signaling Pathway in Endometriosis Rats

目的观察隔药饼灸对子宫内膜异位症大鼠p38MAPK信号通路的干预作用。方法将34只大鼠随机分为A组(空白对照组)8只和造模组26只,造模组大鼠参照子宫内膜异位症造模方法进行造模,然后随机在造模组中选择2只大鼠处死后检验造模结果。将造模成功后的大鼠随机分成B组(隔药饼灸组)、C组(西药组)和D组,每组8只。B组采用隔药饼灸关元穴治疗,C组采用灌服达那唑溶液治疗,D组造模后不予以任何治疗。20 d后处死各组大鼠并取出实验标本。通过荧光定量PCR检测各组样本IFN-?与IL-4的表达;通过Western-blot检测各组样本磷酸化p38MAPK的表达。结果与A组比较,B组、C组和D组大鼠的IL-4m RNA和磷酸化p38MAPK表达显著上升(均P<0.05),IFN-?m RNA表达显著下降(均P<0.05)。与D组比较,B组和C组大鼠的IL-4m RNA和磷酸化p38MAPK表达显著降低(均P<0.05),IFN-?m RNA表达显著上升(均P<0.05)。与B组比较,C组磷酸化p38MAPK、IL-4m RNA和IFN-?m RNA表达无显著性差异(均P>0.05)。结论隔药饼灸能抑制p38MAPK信号通路,抑制IL-4的表达,促进IFN-?生成,诱导Th细胞向Th1分化,恢复Th细胞动态平衡。

Objective To investigate the Intervention effect of herbal cake moxibustion on p38MAPK signaling pathways in endometriosis rats.Methods Thirty-four rats were randomized into group A （blank control） of 8 rats and a model group of 26 rats. A model of endometriosis was made in the model group. Two rats selected randomly from the model group were sacrificed to examine the result of model making. Rats with successful model making were randomized into group B （herbal cake moxibustion）, group C （oral gavage of danazol solution） and group D, 8 rats each. Group A received herbal cake moxibustion on point Guanyuan; group B, an oral gavage of danazol solution; group C, no treatment. After 20 days, every group of rats was sacrificed and the experimental specimens were taken. The expressions of IFN-γ and IL-4 in the specimen were determined by fluorescence quantitative PCR in every group. The expression of phosphorylated p38MAPK was determined by Western blot in every group. Results The expressions of IL-4 mRNA and phosphorylated p38MAPK increased significantly and IFN-γ mRNA expression decreased significantly in groups B, C and D of rats compared with group A （allP〈0.05）. The expressions of IL-4 mRNA and phosphorylated p38MAPK decreased significantly and IFN-γ mRNA expression increased significantly in groups B and C of rats compared with group D （allP〈0.05）. There were no significant differences in the expressions of phosphorylated p38MAPK, IL-4 mRNA and IFN-γ mRNA between groups B and C （allP〉0.05）.Conclusions Herbal cake moxibustion can inhibit p38MAPK signaling pathways and IL-4 expression, promote IFN-γ production, induce the differentiation of Th cells into Th1 cells and restore the dynamic balance of Th cells.