电针对小鼠慢传输型便秘模型结肠动力学影响的神经-内分泌机制研究

Study of the Neuroendocrine Mechanism of the Action of Electroacupuncture on Colonic Motility in a Mouse Model of Slow Transit Constipation

目的通过观察电针干预下慢传输型便秘(slow transit constipation,STC)模型小鼠结肠动力学的效应改变,探讨其神经-内分泌作用机制。方法将40只雌性昆明种小鼠随机分为A组(STC模型电针治疗组)、B组(STC模型电针预防组)、C组(STC模型对照组)和D组(空白对照组),每组10只。A组在造模成功后取上巨虚和大肠俞进行电针治疗;B组于造模开始30 d后介入电针治疗;C组和D组采用相同方法固定,不予针刺。治疗结束后采用碳末推进实验计算肠推进率,并用放免法测定小鼠结肠组织中P物质(SP)和生长抑素(SS)的含量。结果 C组小肠推进率低于D组,两组比较差异有统计学意义(P<0.05);A组和B组肠推进率均优于C组(P<0.05);小鼠结肠组织中,C组SP含量均低于A组、B组和D组,而SS含量则均高于其他3组,差异均有统计学意义(P<0.05)。结论电针可有效改善STC模型小鼠的结肠动力障碍问题。通过调节肠神经-内分泌系统进而改善STC模型小鼠结肠动力障碍是电针治疗STC模型小鼠的主要作用机制。

Objective To investigate the intervention effect of electroacupuncture on colonic motility in a mouse model of slow transit constipation （STC） and explore the neuroendocrine mechanism of its action.Methods Forty female Kunming mice were randomly allocated to groups A （STC model electroacupuncture treatment）, B （STC model electroacupuncture prevention）, C （STC model control） and D （blank control）, 10 cases each. Group A received electroacupuncture at points Shangjuxu and Dachangshu after successful model making; group B, electroacupuncture at 30 days after the beginning of model making; groups C and D, no acupuncture, fastened by the same method. After the completion of treatment, intestinal propulsion rate was calculated by the charcoal powder propelling test and the substance P （SP） and somatostatin （SS） contents of mouse colon were measured by radioimmunoassay.Results Intestinal propulsion rate was lower in group C than in group D; there was a statistically significant difference between the two groups （P〈0.05）. Intestinal propulsion rate was higher in groups A and B than in group C （bothP〈0.05）. The SP content of mouse colon was lower and the SS content was higher in group C than in groups A, B and D; there were statistically significant differences （P〈0.05）.Conclusions Electroacupuncture can effectively reduce colonic motility disorders in a mouse model of STC. The main mechanism of electroacupuncture treatment of slow transit constipation in a mouse model of STC is regulating intestinal neuroendocrine system to reduce colonic motility disorders.