舒脑欣滴丸对自发性高血压大鼠降压及心肌保护作用研究

Effect of Shunaoxin Dropping Pill on blood pressure and cardiac protection in spontaneously hypertensive rats

目的观察舒脑欣滴丸对自发性高血压(SHR)大鼠的降压及心肌保护作用。方法将SHR大鼠按血压和体质量随机分为模型组、舒脑欣滴丸(800 mg/kg)组和阳性药缬沙坦(1 mg/kg)组。以同龄同种系正常血压的京都种(WKY)大鼠作为对照组,各组ig给药,采用无创血压计测定其血压,给药6周后测定大鼠血清中一氧化氮合酶(NOS)和血管紧张素Ⅱ(AngⅡ)的水平,并利用多普勒超声心动检查方法测定左心室质量,利用HE染色观察主动脉和心肌组织形态变化。结果舒脑欣滴丸(800mg/kg)单次给药后,SHR大鼠血压在1h后降到最低,长期给药6周后,可以稳定SHR大鼠的血压;舒脑欣滴丸治疗6周后,大鼠血清中NOS水平显著高于模型组(P<0.05),AngⅡ水平显著低于模型组(P<0.05),舒脑欣滴丸给药组的左心室质量显著低于模型组(P<0.05),且舒脑欣滴丸可改善高血压引起的胸主动脉血管和心肌形态异常。结论舒脑欣滴丸对高血压伴有心肌肥厚大鼠具有一定治疗作用,可作为高血压伴心肌肥厚病症的潜在药物进行研究。

Objective To evaluate the effect of Shunaoxin Dropping Pill （SDP） on blood pressure and cardiac protection in spontaneously hypertensive rats （SHRs）. Methods The SHRs were randomly divided into three groups, model （M） group, SDP group, and valsartan （VL） group. Non-invasive blood pressure instrument was used to measure blood pressure. Serum content of nitric oxide synthase （NOS） and angiotensin II （Ang II） were measured using kits for 6 weeks after ig administration. Doppler echocardiography was used to evaluate left ventricular weight, and HE staining was used to assess aortic and cardiac morphology. Results The blood pressure of SHRs decreased to minimum 1 h after ig administration of SDP （800 mg/kg）. Long-term use of SDP could stabilize blood pressure of SHRs. Serum content of NOS in SDP group was significantly more than that in M group （P 〈 0.05）. The serum content of Ang II in SDP group was significantly less than that in M group （P 〈 0.05）. The left ventricular weight of SDP group was significantly lower than that in M group （P 〈 0.05）. SDP administered group can improve myocardial and aortic morphology abnormalities caused by hypertension. Conclusion SDP has a certain therapeutic effect on hypertensive with myocardial hypertrophy rats, it can be used as a potential drug on hypertensive disorders associated with cardiac hypertrophy.