半夏与其混伪品掌叶半夏SCAR标记鉴别研究

Study on SCAR Markers to Identify Pinellia ternata and Pinellia pedatisecta

目的:建立快速准确的半夏和掌叶半夏分子标记鉴定方法。方法:通过RAPD随机引物的筛选,获得半夏特异的RAPD分子标记片段,经克隆、测序,重新设计特异性引物转化形成稳定的SCAR标记。结果:获得1条稳定扩增的半夏特异片段T350,根据该特异片段序列设计1对特异引物,分别对半夏和掌叶半夏DNA进行扩增,引物可从半夏DNA中扩增获得约330bp的片段,而掌叶半夏未扩增出该片段。结论:T350成功转化为SCAR分子标记,可对半夏和掌叶半夏进行快速有效的鉴定。

Objective ： To establish sequence characterized amplified region markers（SCAR） of Pinellia ternate. Methods. The random primer was screened through RAPD to obtain the specific RAPD marker band, and the band was separated, extracted, cloned and sequenced. The specific primers were designed for conventional PCR reaction on the basis of the specific band, and the specific SCAR marker of P. ternata was acquired. Results. The results of PCR showed that a 350 bp electrophoresis band of P. ternata named T350 was found. And a specific primer was designed and used to identify P. ternata and P. pedatisecta effectively. Conclusion：The RAPD marker T350 was successfully converted into SCAR marker. It was an effective way to identify P. ternata and P. pedatisecta more rapidly.