重瓣大岩桐离体培养的两种途径

Two Pathways for Isolated Culture of Sinningia speciosa

以大岩桐的茎段、叶片、茎尖为外植体,以MS为基本培养基,附加不同浓度的激素组合进行离体培养研究。结果表明：适宜外植体灭菌的方法为先用75%的乙醇表面消毒10 s后再用0.1%Hg Cl2灭菌4~5 min,或者先用75%的乙醇表面消毒5 s后再用0.1%Hg Cl2灭菌6 min。3种外植体均可获得组培苗,叶片通过愈伤组织诱导途径可获得组培小苗;茎段和茎尖通过不定芽途径可获得组培小苗。最适宜的愈伤分化培养基为MS＋6-BA 0.50 mg/L＋IBA 1.00 mg/L,适宜的不定芽增殖培养基为MS＋6-BA 0.50 mg/L或者MS＋6-BA 0.50 mg/L＋IBA 1.00 mg/L,适宜的生根培养基为MS＋IBA1.00^2.00 mg/L,降低蔗糖含量有利于生根。MS＋IBA 2.00 mg/L＋AC 0.10 g/L有利于生根培养。

The leaf,shoot tip and stem of Sinningia speciosa were used as explants,and their isolated culture was conducted on the MS medium supplemented with different concentrations of plant hormones. The best sterilization conditions for explants were obtained as follows： 75% alcohol for 10 s plus 0.1% Hg Cl2 for 4 ~ 5 min,or 75% alcohol for 5 s plus 0.1% Hg Cl2 for 6 min. Tissue-culture seedlings could be obtained from the explant leaf through callus induction pathway,and could also be obtained from the explants stem and shoot tip through adventitious bud multiplication pathway. The best culture medium for callus differentiation was MS＋ 6-BA 0.50 mg/L＋IBA 1.00 mg/L; the best culture medium for adventitious bud multiplication was MS＋ 6-BA 0.50 mg/L or MS＋ 6-BA 0.50 mg/L＋IBA 1.00 mg/L; the optimum culture medium for rooting was MS＋IBA 1. 00 ~ 2. 00 mg/L,and low concentration of sucrose in MS was advantageous to rooting; the medium MS＋IBA 2.00 mg/L＋AC 0.10 g/L was beneficial to the culture of rooting.