抗阻和被动拉伸联合训练对大鼠腓肠肌卫星细胞激活相关因子基因表达的影响

Effects of Combination of passive Stretching and Resistance Exercise on HGF and MGF mRNA of Rat's Gastrocnemius

目的:通过大鼠运动实验模型,观察抗阻和被动拉伸训练后大鼠腓肠肌HGF、MGF及其基因表达的变化,研究被动拉伸和抗阻联合训练对骨骼肌卫星细胞激活和增殖的影响;方法:32只成年健康SD大鼠随机分为4组,对照组(N组,n=8)、被动拉伸组(S组,n=8)、抗阻组(R组,n=8)和混合组(C组,n=8)。N组不做任何处理,对S组和C组大鼠右侧腓肠肌手动进行被动拉伸训练,每分钟15次,每次维持2s,每天15min,每周4次,共10周。对R组和C组进行尾部负重爬梯训练,负重为体重的200%,每周3天,每天2组,每组3次,共10周。训练结束后取右侧腓肠肌,测定腓肠肌湿重,ELISA法测定MGF,HGF,RT-PCR测定MGF、HGF的基因表达。结果:训练组实验后各项指标均有显著上升,其中联合训练组各项指标上升最为明显,与N组比较差异非常显著(P<0.01),与R组相比较除HGF外其余指标也差异显著,但HGF mRNA较S组却无明显差异;S组各项指标虽也有显著上升,但较R组上升幅度要低,且差异非常显著;结论:被动拉伸训练和抗阻训练都可以有效上调,HGF、MGF及其mRNA的表达,进而激活肌卫星细胞进入细胞周期分裂增殖,且两种训练对卫星细胞激活的效果可以叠加。

Objective： To investigate the effect of the combination of passive stretching and resistance exercise on the activation of skeletal muscle satellite cell,through examine the expression of HGF、MGF mRNA of rat＇s gastrocnemius. Methods： 32 adult SD rats were randomly divided into 4 groups： control group（ group C,n = 8）,nothing were done; stretch group（ group S,n = 8）,animals right gastrocnemius muscle was stretched repetitively for 2 seconds / time,15 times / min,15 min daily and 4times / week under anesthesia; resistance group（ group R,n = 8）,animals underwent 10 weeks（ 3 times / set,2sets / day,3days / week） climbing ladder training with weights（ as 200% of their body weights） attached to the rats tails; combination group（ group C,n = 8）,animals underwent both stretching and resistance trains for 10 weeks. After training,animal ＇s the right gastrocnemius were token respectively. The protein HGF、MGF were detected by the ELISA,RT-PCR was applied to detect the expression of HGF mRNA and MGF mRNA in gastrocnemius muscle. Results： After the combination of passive stretching and resistance exercise,the weight of gastrocnemius muscle,the expression of HGF,MGF and mRNA was higher in group S,R,C than the group N（ P〈0. 05）. Compare to the group N,the expression of HGF,MGF and their mRNA was most in the group C（ P〈0. 01）. Compare to group R,the expression of HGF mRNA,MGF mRNA and MGF was high significant differences（ P〈0. 05）. But compare to the group S,the expression of HGF mRNA of the group C was not significant differences; Compare to the group R,the expression of HGF,MGF and their mRNA was high significant differences in group S.Conclusion： Both passive stretching and resistance exercise can effectively improve the expression level of the HGF,MGF and their mRNA,and activate the satellite ecll into the cell cycle to differentiation and proliferation. And the effect of them can be accumulated.