超高效液相色谱法测定虾青素

Determination of Astaxanthin by Ultra Performance Liquid Chromatography

建立超高效液相色谱法快速检测虾青素的方法.采用UPLC BEH C8 色谱柱（50 mm ×2.1 mm,1.7 μm）,考察了流动相、流量及柱温对虾青素样品分离的影响,确定了最佳色谱条件等度洗脱,流动相为甲醇– 水（ 体积比为75∶25）,流量为0.5 mL/min,柱温为40℃,检测波长为475 nm.虾青素的质量浓度在0.2～10.0 μg/mL 范围内与其色谱峰面积呈良好的线性关系,线性相关系数r=0.998 8,检出限（S/N=3） 为0.1 μg/mL,定量限（S/N=10） 为0.2μg/mL.测定结果的相对标准偏差为0.41%（n=6）,加标回收率为105.8%～110.3%.该方法快速、简单、可靠、灵敏、重复性好,可用于虾青素有关样品的快速检测.

A ultra performance liquid chromatography（UPLC） method for determination of astaxanthin wasdeveloped. The determination of astaxanthin was performed on a UPLC BEH C8 column （50 mm×2.1 mm,1.7 μm）. Theinfluence of mobile phase,flow rate and column temperature on the separation of astaxanthin was comprehensively studied.The optimal separation condition was as follows the mobile phase was methanol–water （volume ratio was 75∶25） with aisocratic elution profile and flow rate of 0.5 mL/min,the detection wavelength was 475 nm, the column temperature was40℃. The mass concertration of astaxanthin has good linear ralationship with the chromatographic peak area in the range of0.2–10.0 μg /mL, the correlation coefficient r was 0.998 8. The detection limit was 0.1 μg/mL （S/N=3）. The quantitationdetection limit was 0.2 μg/mL（S/N=10）. The relative standard deviation of determination results was 0.41%（n=6）, and therecovery was 105.8%–110.3%. The method is rapid,simple,reliable,and sensitive with a good reproducibility, it can beused for the determination of astaxanthin.