呼吸道铜绿假单胞菌感染病灶藻酸盐合成相关基因的表达

Expression of alginate biosynthesis-related genes in respiratory Pseudomonas aeruginosa infection lesions

目的:获得一种呼吸道铜绿假单胞菌生物膜检测方法,探讨藻酸盐合成相关基因的表达对临床治疗的影响。方法:提取60例呼吸道铜绿假单胞菌感染患者气管内病变组织,实时荧光定量PCR检测藻酸盐合成相关基因algD、algB、algU、mucA的表达,60例患者根据是否耐药分组,分为耐药组(R组)40例,非耐药组(NR组)20例,根据治疗的难易程度,将60例患者分为治疗困难组(D组)38例,治疗容易组(E组)22例。并进行基因algD、algB、algU、mucA的表达与临床抗生素耐药及治疗难易之间的相关性分析。结果:经细菌培养证实的铜绿假单胞菌感染病例60例,呼吸道病变组织均有algD、algB、algU、mucA基因的表达;耐药组(R组)和非耐药组(NR组)对比,algD、algU表达有统计学差异,algB、mucA表达无统计学差异;容易组(E组)和困难组(D组)对比,algD、algU、algB、mucA差异无统计学意义;细菌耐药和治疗难易的相关性分析显示,细菌耐药和临床治疗难易相关。结论:algD与铜绿假单胞菌藻酸盐的合成有着直接的正相关性,可以作为判断是否有黏液型铜绿假单胞菌生长及生物膜形成的依据,而algU与algD的表达有正相关性。

Objective ：To obtain a respiratory Pseudomonas aeruginosa biofilm detection methods ,explore the affect of the expression of the related alginate synthesis genes to the clinical treatment .Methods ：Specimens were taken from the tracheal lesions of Pseudomonas aeruginosa infection cases ,real-time quantitative PCR was performed to detect the expression of alginate biosynthesis-related gene algD ,algB ,algU ,mucA .And correlation between gene algD ,algB ,algU ,mucA .Expression and clinical treatment effect and antibiotic resistance was statistically analysed .Results ：For all of the bacterial culture confirmed Pseudomonas aeruginosa infection cases ,the alginate biosynthesis-related gene Expression was detected ;comparison of resistant group （R group） and non-resistant group （NR group） ,algD ,algU expression significantly difference ,algB ,mucA difference was not significant .Conclusion ：Expression of algD and Pseudomonas aeruginosa alginate synthesis has a direct positive correlation .Detect of algD can be used to determine whether there is Pseudomonas aeruginosa biofilm formation .and the expression of algU have a positive correlation with algD .