^(60)Coγ射线对雄性比格犬睾酮分泌及睾丸组织病理学的影响

Effects of ^(60)Co γ rays on testosterone secretion and testicular histopathology of male Beagle dogs

目的:探讨^(60)Coγ射线急性照射对雄性比格犬睾酮分泌的影响及对睾丸的损伤。方法:检疫30 d的15只雄性比格犬,随机分为3组:空白对照组、0.5Gyγ射线照射组和5.0Gyγ射线照射组,照射组比格犬接受^(60)Coγ射线一次性全身照射,吸收剂量率为0.5Gy/min,分别于照射后第4、7、14、28、60天采用竞争性抑制酶免疫分析法测定血清睾酮含量,照射后60 d取犬睾丸和附睾,观察脏器是否出血或坏死并进行睾丸的组织结构病理学检查。结果:γ射线照射后10~13 d,5.0 Gyγ射线照射组动物全部死亡,并出现睾丸及附睾出血;照射后60 d,0.5 Gyγ射线照射组动物体质量与空白对照组间的差异无统计学意义(P>0.05),其睾丸平均质量为空白对照组的69%,两组间的睾丸,体质量比差异无统计学意义(P>0.05);在照射后第4、7、14、28、60天,0.5和5.0Gyγ射线照射组与空白对照组血清睾酮含量间的差异无统计学意义(P>0.05);2个照射组比格犬睾丸生精细胞有不同程度坏死脱落,其中5.0Gyγ射线照射组睾丸内无精子生成。结论:0.5和5.0Gy^(60)Coγ射线急性照射均对雄性比格犬生殖系统造成损伤,其中血清睾酮变化不明显,睾丸结构受到破坏,生精细胞及精子生成受到严重损伤。

OBJECTIVE：To investigate the effects of ^60Co γ rays on testosterone secretion and testicular histopathology of male Beagle dogs.METHODS：15 male Beagle dogs were subdivided into 3 groups：the control group and the irradiation groups.Irradiation groups were exposed to 0.5 Gy or 5.0 Gy ^60Co γ ray,dosage rate 0.5 Gy/min.At the4^th-,7^th-,14^th-,28^th- and 60^th-day after irradiation,the content of serum testosterone was determined by the competitive inhibition immunoassay.And at 60^th day after irradiation,the testicles were obtained for observation and histopathology.RESULTS：During 10-13 days after irradiation,all the animals of the 5.0 Gy γ ray irradiation group died,and hemorrhage spots were found on their testes and epididymises.At 60^th day after irradiation,there was no difference statistically between the 0.5 Gy γ ray irradiation group and the control group.The testicle average weight of 0.5Gy group was 69%of the control group,while there was no statistical difference for testicle/body weight ratio between the0.5 Gy γ ray irradiation group and the control group.Meanwhile,at 4^th-,7^th-,14^th-,28^th-and 60^th-day after irradiation,there was no statistical difference for serum testosterone content among the 0.5 Gy γ ray irradiation group and blank control group.Spermatogenic cells from the 0.5 Gy and 5.0 Gy γ ray irradiation groups showed necrosis and lysis.In addition,azoospermia generation wasn＇t found in the testicle of the 5.0 Gy group.CONCLUSION：Acute 0.5 Gy and5.0 Gy γ ray irradiation induced damage to the reproductive system of male Beagle dogs：the testicle structure,spermatogenic cells and azoospermia generation were severely damaged.However,serum testosterone content did not show much obvious changes,