三氯乙烯药疹样皮炎患者血清miRNAs差异表达谱分析

Differentially expressed miRNA profile in workers with trichloroethylene-induced

目的:分析三氯乙烯药疹样皮炎(OMLDT)患者和三氯乙烯接触者血清micro RNA(mi RNA)表达谱的差异。方法:分别采集5名三氯乙烯药疹样皮炎患者和5名接触者血液,分离血清,抽提总RNA进行Hy3荧光标记,然后浓缩标记的样品,运用mi RCURY LNA芯片进行mi RNA芯片杂交,杂交后进行图像扫描,所得数据使用Gene Pix Pro V6.0软件进行mi RNA差异表达分析并采用q PCR进行验证。对差异较大的mi RNA用mi RWalk 2.0进行靶基因预测,并与其他预测软件结果进行比较。结果:OMLDT患者与三氯乙烯接触者比较,共有69个差异表达的mi RNAs,其中低表达30个,高表达39个(包含mi R-199b)。q PCR验证亦显示mi R-199b在OMLDT中高表达。靶基因预测结果显示mi R409-3p和mi R485-3p在5个预测软件均预测到了共同的靶基因。结论:三氯乙烯药疹样皮炎患者血清mi RNAs表达谱发生了显著改变,mi R-199b可能是OMLDT潜在的易感生物标志物。

OBJECTIVE：To profile microRNAs expression among workers with trichloroethylene（TCE）-induced medicamentosa-like dermatitis（OMLDT）.METHODS：Serum samples from 5 patients with OMLDT and with TCE contacts were collected.The total RNA was extracted then labeled with Hy3 fluorescent.MicroRNAs were hybridized with miRCURY LNA chips and images were acquired using the GenePix 4000 B scanner,qPCR method was used to verify the results of miRNA microarray.The results were further analyzed by prediction softwares.RESULTS：A total of 69 microRNAs were differentially expressed between OMLDT and TCE contacts.Among the microRNAs,39 were upregulated and 30 were down-regulated.Increase of miR-199b-5p was verified using qPCR.Target prediction results showed that the same target genes of miR-409-3p and miR-485-3p had been predicted in all forecasting software.CONCLUSION：The study showed that expression of microRNA in serum of workers with OMLDT was significantly altered,and miR-199 b might serve as a candidate biomarker for OMLDT.