花叶矢竹转录组中的转座子表达分析

Transposons expression analysis of transcriptome in Pseudosasa japonica f. akebonosuji

以花叶矢竹Pseudosasa japonica f.akebonosuji 10种不同颜色和不同发育阶段的叶片转录组数据为基础,调查了花叶矢竹中转座子的种类、数量以及选择性表达特性。结果表明:花叶矢竹转录组中转座子类型丰富、数量繁多,其中RNA转座子明显多于DNA转座子,转座子LTR/Copia类型数量最多。绿叶的5个发育阶段中,转座子主要在第5发育阶段高表达,表明这些转座子可能参与了花叶矢竹叶片成熟过程。而在白叶5个发育阶段中,转座子主要在第1发育阶段高表达;对绿叶和白叶5个相对应的发育阶段分析表明,白叶中高表达的转座子多于绿叶,表明这些转座子可能参与了花叶矢竹叶色变异过程,也可能是逆境胁迫导致转座子转座激活。

To study the super- family, abundance, and expression patterns of transposons in Pseudosasa japonica f. akebonosuji, transcriptome data from 10 kinds of different colors and different leaf developmental stages were assembled. Transposons were identified by Protein-based RepeatMasking. Then a comparison analysis of trans-posons for the five corresponding developmental stages of green leaves and albino leaves was conducted. Results showed many different super-families of transposons in transcriptome with more RNA （74.06% ） than DNA （23.36%） transposons and with LTR/Copia transposons （25.38%） the most abundant for all super- fami-lies. Among the five developmental stages of green leaves, the fifth developmental stage had the most high-level expressed transposons （49.48%）. Also, among the five developmental stages of albino leaves, the first develop-mental stage had the most high-level expressed transposons. The comparison analysis of the five corresponding developmental stages of green and albino leaves showed more high-level expressed transposons in albino leaves （55.14%）. Thus, in green leaves of P. japonica f. akebonosuji, transposons could be involved with leaf maturation; also to lay a foundation for whether transposons regulate leaf color variation, the green and albino leaf comparison revealed that transposons could influence leaf color variation with high expression of transposons due to stress from abnormal photosynthesis. ＆Ch, 3 fig. 3 tab. 25 ref.]