摘要
目的研究胃癌组织中核因子E-2相关因子2（nuclear factor E-2 related factor,Nr F-2）和血红素氧合酶-1（heme oxygenase-1,Ho-1）的表达并探讨其临床意义。方法选取从2016年2月-2016年6月,杭州市肿瘤医院病理科存档的人胃组织蜡块176例,其中胃癌78例,正常组织78例。分别采用免疫组织化学法检测2组人胃组织中Nr F-2和Ho-1的表达情况并进行分析。结果胃癌组Nr F-2和Ho-1水平分别为（0.752±0.098）、（0.862±0.081）,均显著高于正常组的（0.381±0.068）、（0.412±0.083）;Nr F-2、Ho-1阳性表达率在胃壁侵犯程度为T3＋T4、伴有淋巴结转移、NTM分期为Ⅲ、Ⅳ期胃癌组织中显著增高,上述差异均有统计学意义（均P〈0.05）。结论 Nr F-2和Ho-1在胃癌的发生发展中具有重要作用,且Nr F-2、Ho-1在胃癌组织中的阳性表达率与胃壁侵犯程度、有无淋巴结转移、NTM分期存在密切相关。提示了临床诊断中若联合检测Nr F-2和Ho-1,有利于对胃癌的早期诊断以及预后。
Objective To study nuclear factor E-2 related factor 2（NrF-2） and heme oxygenase-1（Ho-1） expression and to explore its clinical significance. Methods From February 2016 to June 2016,176 cases human gastric tissue paraffin blocks selected in department of pathology in Hangzhou Cancer Hospital,including 78 cases of gastric cancer,78 cases of normal tissue were analyzed. And the expressions of NrF-2 and Ho-1 were detected by immunohistochemistry in two groups of human gastric tissue. Results The levels of NrF-2 and Ho-1 in gastric cancer group were（0. 752 ±0. 098）,（0. 862 ± 0. 081） were significantly higher than those in normal group（0. 381 ± 0. 068）,（0. 412 ± 0. 083）; NrF-2,the positive expression rate of Ho-1 in gastric wall invasion degree of T3 ＋ T4 with lymph node metastasis,NTM stage III and IV gastric cancer tissues were significantly increased. The differences were statistically significant（P〈0. 05）. Conclusion NrF-2 and Ho-1 play the important role in the development of gastric cancer and NrF-2,the positive expression rate of Ho-1 in the gastric carcinoma and gastric wall invasion,lymph node metastasis and NTM staging are closely related. The clinical diagnosis if the combined detection of NrF-2 and Ho-1,is conducive to the early diagnosis of gastric cancer and the prognosis of the disease.
出处
《中国生化药物杂志》
CAS
2016年第9期146-148,共3页
Chinese Journal of Biochemical Pharmaceutics
