MeJA处理对蓝莓果实采后灰霉病的影响及机理

Effect and Mechanism of Methyl Jasmonate on Incidence of Grey Mould Decay in Postharvest Blueberry

以"埃利奥特"蓝莓果实为实验材料,研究茉莉酸甲酯(methyl jasmonate,Me JA)处理对蓝莓果实灰霉病、活性氧代谢酶、防御相关酶活性和总酚、总花色苷含量的影响。50μmol/L Me JA处理蓝莓果实后刺孔接种Botrytis cinerea孢子悬浮液,于(1±1)℃贮藏16 d。结果表明,Me JA处理有效抑制果实发病率和过氧化氢酶、抗坏血酸过氧化物酶活性,促进贮藏初期果实H2O2的迅速积累,提高果实防御相关酶苯丙氨酸解氨酶、过氧化物酶和多酚氧化酶活性,促进酚类、木质素等植保素类物质的合成。表明Me JA处理可提高蓝莓果实抗病性,从而有效抑制了果实灰霉病的发生。

The effects of methyl jasmonate（Me JA） treatment on postharvest grey mould decay, the activities of enzymes involved in reactive oxygen species（ROS） metabolism, defense-related enzymes activities and total phenol and total anthocyanin contents in ‘Elliott＇ blueberry were investigated. Blueberry fruits were treated with Me JA at 50 μmol/L, inoculated with Botrytis cinerea and then stored at（1 ± 1） ℃ for 16 days. Me JA treatment resulted in significantly lower disease incidence compared with control fruits. Me JA treatment significantly inhibited the activities of catalase（CAT） and ascorbate peroxidase（APX） and thus resulted in a higher level of H2O2 in the early period of storage. However, Me JA treatment enhanced the activities of phenylalanine ammonialyase（PAL）, polyphenoloxidase（PPO） and peroxidase（POD）, and induced the synthesis of phenolics and lignin. These results suggest that Me JA treatment can reduce the incidence and severity of grey mould decay by enhancing the disease resistance of blueberry fruit.