103个杂交稻亲本白叶枯病和稻瘟病抗性基因型检测

Genotyping of 103 Parents of Hybrid Rice Using DNA Markers for Bacterial Blight and Blast Resistance Genes

白叶枯病和稻瘟病是我国水稻生产的主要病害,选育抗病品种是一种经济有效的防治手段。明确抗性基因在水稻品种资源中的分布,将有助于应用分子标记辅助选择培育抗性品种。本研究针对13个重要抗病基因,包括8个白叶枯病抗性基因(Xa1、Xa4、xa5、Xa7、xa13、Xa21、Xa23和Xa26)和5个稻瘟病抗性基因(Pib、Pi2、Pi9、Pi25和Pita),应用这些基因的基因标记或连锁标记共29个,检测103份杂交稻亲本,初步确定了其等位基因分布和利用情况,为水稻基础材料的筛选及应用提供参考。

The rice blast and bacterial blight are two of the most serious disease in rice production. The use of resistance genes in rice breeding has been known to be an efficient approach for rice protection, which requires an understanding the allelic distribution of the resistance genes in rice germplasm. The objective of this study is to determine the allelic variation of 13 resistance genes in 103 parental lines of hybrid rice,including 8 genes for bacterial blight resistance （Xal, Xag, xaS, XaT, xal3, Xa21, Xa23 and Xa26） and 5 ones for blast resistance （Pib, Pi2, Pi9, Pi25 and Pita）. 29 DNA markers determining the 13 genes were used for genotyping. The information could be useful in the processing of variety improvement.