启明丸对白内障模型大鼠MDA、SOD、GSH-Px、G-6-PD水平及晶状体蛋白含量变化的影响

Effect of Qiming Pill(启明丸) on MDA, SOD, GSH-Px, G-6-PD Levels and Lens Protein Content in Cataract Model Rats

目的:观察启明丸对白内障模型大鼠MDA、SOD、GSH-Px、G-6-PD水平及晶状体蛋白含量变化的影响。方法:将50只SD大鼠按照随机数字表分为5组,对照组,模型组及启明丸低、中、高剂量组;采用亚硒酸钠造模,造模成功后予相应药物灌胃,灌胃28 d后,摘取大鼠双侧眼球晶状体,匀浆,离心,分离上清液,测定MAD、SOD、GSH-Px和G-6-PD水平及晶状体匀浆蛋白含量。结果:启明丸中、高剂量组MDA及晶状体匀浆蛋白含量均低于模型组,SOD、GSH-Px、G-6-PD水平均高于模型组(P<0.05);启明丸中、高剂量组与低剂量组比较,差异均有统计学意义(P<0.05)。启明丸中、高剂量均能够调整MDA、SOD、GSH-Px、G-6-PD水平,降低晶状体中蛋白含量。结论:启明丸能调整白内障模型大鼠晶状体MDA、SOD、GSH-Px、G-6-PD水平,降低晶状体中蛋白含量,从而改善晶状体混浊程度。

Objective： To observe the effect of Qiming Pill on MDA model, SOD, GSH-Px, G-6-PD level and lens protein in cataract model rats. Methods： The 50 SD rats were randomly divided into 5 groups： the control group, model group and Qiming low, medium and high dose groups. The model was made by sodium selenite, and after successful modeling, the groups were given corresponding drugs intragastric administration. After 28 days, the lens of the rats were homogenized, centrifuged, and the superuatant was separated to measure MAD, SOD, GSH-Px and G-6-PD lens homogenate levels and protein content. Results： MDA and lens homogenate protein content of Qiming pills high and medium dose group were lower than the model group, while SOD, GSH-Px, G-6-PD lens were higher than the model group （P〈0.05）, and compared with the low dose group, the differences were statistical significance （P〈0.05）. Qiming pills high and medium dose can regulate the levels of MDA, SOD, G-6-PD and GSH-Px, and reduce the protein content of the lens. Conclusions： Qiming pills can adjust the MDA, SOD, GSH-Px, G-6-PD levels and reduce lens protein content, then to improve the degree of lens opacity.