芍药苷对大鼠皮质酮损伤的海马神经元TrkB/BDNF信号通路的影响

Effect of Paeoniflorin on the TrkB/BDNF signaling pathway of corticosterone-damaged hippocampal neurons

目的观察芍药苷对皮质酮诱导海马神经元损伤的影响。方法体外培养新生SD大鼠海马神经元,采用皮质酮诱导原代培养海马神经元建立皮质酮损伤模型。培养体系中加入不同浓度的芍药苷进行预处理。采用WST-1法检测芍药苷对神经元存活率的影响,应用PCR与Western印迹检测神经元脑源性神经营养因子(BDNF)与酪氨酸激酶受体B(Trk B)的表达。结果与皮质酮组相比,中高剂量芍药苷可明显增加海马神经元活力(P<0.05),可明显增加海马神经元Trk B与BDNF的mRNA与蛋白表达水平(P<0.05)。结论芍药苷对皮质酮诱导的原代培养海马神经元损伤具有保护作用,具有抗抑郁作用。

Objective To observe the effect of paeoniflorin on the damage of hippocampal neurons induced by corticosterone and in- vestigate the mechanisms of paeoniflorin against depression. Methods Hippocampal neurons derived from newborn SD rats were cultured in vitro, using primary cultured hippocampal neurons induced by corticosterone to set up the corticosterone-injured model. The culture system was added to different concentrations of corticosterone pretreatment. WST-1 assay was used to detect the effect of paeoniflorin on the neuronal sur- vival rate. The expression of TrkB/BDNF was detected by PCR technology. Results Compared with the model group, the medium and high dosage of corticosterone could significantly increase the hippocampal neuronal viability. The differences were significant（P〈0.05 ）. The medi- um and high dosage of corticosterone could obviously enhance the mRNA expression level of TrkB/BDNF（P〈0.05）. Conclusions Paeoni- florin has protective effect on corticosterone-induced primary cultured hippocampal neurons and antidepressant effect.