鼻咽拭子EB病毒DNA检测在鼻咽癌诊断中的价值

Value of Epstein-Barr virus DNA detection in nasopharyngeal swabs for diagnosis of nasopharyngeal carcinoma

目的探讨EB病毒(EBV)-DNA检测方法及其在鼻咽癌(NPC)早期诊断中的价值。方法应用聚合酶链反应(PCR)荧光定量检测185例疑诊NPC患者鼻咽拭子及外周血EBV-DNA,并进行比较分析。185例疑诊NPC患者最终病理确诊为NPC 141例(NPC组),其余44例为非NPC组。结果 2组患者鼻咽拭子EB病毒DNA检测的阳性率均较外周血检测的阳性率高,差异有统计学意义;鼻咽拭子EBV-DNA检测在2组间差异有统计学意义(P<0.05);外周血EBV-DNA检测在2组间差异无统计学意义(P>0.05)。结论鼻咽拭子EBV-DNA检测较外周血更为敏感,有助于NPC的早期诊断。

Objective To explore the detection method of Epstein-Barr virus （EBV）-DNA and its value in the early diagnosis of nasopharyngeal carcinoma （NPC）. Methods Polymerase chain reaction （PCR） was used to detect the EBV-DNA both in the nasopharyngeal swab and in the peripheral blood in 185 patients suspected as nasopharyngeal carcinoma, in which 141 cases were pathologically diagnosed as NPC（ NPC group） and the other 44 cases as non NPC （non NPC group）. Results The objective rate of EBV DNA detected in nasopharyngeal swabs was higher than that in peripheral blood both in NPC group and in non NPC group. However, EBV was highly expressed in nasopharyngea] swabs than in peripheral blood in nasopharyngeal swabs （P 〈 0.05 ）, while the difference of the objective expression of EBV- DNA between the two groups was not significant in peripheral blood （P 〉 0.05）. Conclusions It＇ s more useful for the early diagnosis of NPC to detect EBV in nasopharyngeal swabs than in peripheral blood. （ Chin J Ophthalmol and Otorhinolaryngo1,2016,16：385-387 ）