恶性黑素瘤组织COX-2与HIF-1α表达及意义

Value of COX-2 and HIF-1α expression in malignant melanoma

目的检测恶性黑素瘤组织中环氧化酶2(COX-2)与缺氧诱导因子-1α(HIF-1α)的表达,以明确其在恶性黑素瘤发生、发展中的临床意义。方法收集40例恶性黑素瘤和20例色素痣组织,采用免疫组化方法检测组织中COX-2与HIF-1α的表达并进行相关分析。结果 40例黑素瘤患者中33例(82.5%)COX-2(+),高于对照组(15%)(P<0.01)。黑素瘤患者中35例(87.5%)HIF-1α(+),高于对照组(10%)(P<0.01)。恶性黑素瘤患者组织中COX-2与HIF-1α表达存在正相关(r=0.324,P<0.05)。恶性黑素瘤HIF-1α表达在年龄、性别和部位分组中,差异不显著(P>0.05)。而恶性黑素瘤患者中病理分期高,且有淋巴结转移者则HIF-1α阳性率高(P<0.05)。结论 HIF-1α参与恶性黑素瘤的进展,可能与COX-2协同作用。

Objective To explore the expression of COX-2 and HIF-1α and their clinicopathological value in malignant melanomas （ MM ）. Methods The clinical data of 40 patients with MM and 20 with nevus were collected. The expression of COX-2 and HIF-1α was detected by immunohistochemical method. Rseults The positive rate of COX-2 （33/40, 82. 5% ） and HIF-1α （35/40, 87. 5 % ）in MM was higher than those of the control group （P 〈 0.01 ）. There was a statistically positive correlation between the expression of COX-2 and HIF-lct in MM （r = 0. 324, P = 0. 03）. The expression of HIF-1α was not related with age, sex and sites （ P 〉 0. 05 ）. The expression of HIF-1α had a higher positive rate in malignant melanoma tissue when the patients was in advanced pathological stage and with lymph node metastasis （P 〈 0. 05 ）. Conclusion HIF-loL can be involved in the development of MM. It may cooperate with COX-2.