瞬时受体电位通道M2外湿子单核苷酸多态性rs1556314与脓毒症的相关性分析

Association of single nucleotide polymorphism in exon of transient receptor potential melastatin 2 gene with sepsis

目的：探讨瞬时受体电位通道M2（ TRPM2）外显子单核苷酸多态性rs1556314与脓毒症发生、发展的相关性。方法：采用病例对照研究方法，将浙江大学医学院附属第一医院的119例脓毒症患者和112例对照者纳入本研究中。其中，脓毒症患者按照住院28 d转归不同分为存活组（57例）和死亡组（62例）。采用PCR-TaqMan探针技术检测TRPM2第11个外显子rs1556314基因型，分析其与脓毒症易感性及预后的关系。结果：TRPM2第11个外显子rs1556314基因型GG、GT、TT的分布频率脓毒症组与对照组之间差异无统计学意义（ P＞0．05），等位基因G、T的分布频率两组间差异亦无统计学意义（P＞0．05）。在脓毒症死亡患者与存活患者中TRPM2第11个外显子rs1556314基因型GG、GT、TT和等位基因G、T分布频率差异均无统计学意义（均P＞0．05）。结论：本研究结果提示TRPM2第11个外显子rs1556314的基因型和等位基因与脓毒症发生、发展无相关性，尚需大样本实验数据进一步证实。

Objective： To investigate the association between single nucleotide polymorphism （ SNP ） in the 11th exon of transient receptor potential melastatin 2 （TRPM2） gene with the susceptibility and outcome of sepsis.Methods：A total of 119 septic patients and 112 normal subjects were enrolled from the First Affiliated Hospital, Zhejiang University School of Medicine.Among 119 septic patients, 62 died （ fatal group） and 57 survived （survival group） within 28 days of disease onset.The genotypes of these individuals were detected using TaqMan allelic discrimination assays, and its correlations with susceptibility and outcome of sepsis were analyzed.Results：There was no significant difference in genotype frequencies and allelic frequencies of TRPM2 SNP rs1556314 between septic patients and the controls （all P〉0.05）.And no significant difference in genotype frequencies and allelic frequencies of TRPM2 SNP rs1556314 was observed between the survivors and fatal cases of septic patients （ all P 〉0.05 ） . Conclusion： The TRPM2 SNP rs1556314 does not have significant association with sepsis, but this result need to be confirmed by large scale studies.