利用miR-17的腺病毒载体抑制肺癌细胞系增殖

Expression of miR-17 via Adenoviral Vector Suppress the Proliferation of A549 Cells

miR-17是肺癌的新型调节因子,有可能在肺癌发生和进展中发挥作用,但其功能尚不完全清楚。构建miR-17的腺病毒表达载体,检测miR-17对A549细胞体外增殖、锚定非依赖性生长、侵袭和转移的调控作用。利用miR-17的腺病毒载体(Ad-miR-17)在A549细胞中过表达miR-17后,使用MTT实验检测miR-17对NSCLC细胞系A549增殖作用的影响;软琼脂成集落实验检测miR-17对A549细胞锚定非依赖性生长的作用,使用Trans-well实验检测A549细胞的体外侵袭/转移作用(in vitro invasion/in vitro migration);在此基础上共转染miR-17的inhibitor确定miR-17作用的特异性。结果表明,miR-17能够显著下调A549细胞的增殖、锚定非依赖性生长、体外侵袭/转移作用;共转染miR-17的inhibitor能够阻断miR-17的作用。

MicroRNA-17 （miR-17） may participate in the regulation of human lung cancer regulation or pro- gress. However, the detailed function and mechanisms of miR-17 are largely unknown. To construct the adenoviral vector of miR-17 and examine its roles in lung cancer cells＇ proliferation, anchorage independent growth, in vitro invasion and migration. Full length sequence of miR-17 was cloned into recombinant adenovirus vectors. A549 cells were infected with adenovirus vectors （Ad-miR-17 vectors）. The proliferation, anchorage-independent growth in vitro invasion or migration of A549 cells were determined by MTT-assays, soft agar-assays or trans-well assays. The specificity of miR-17 function was confirmed by transfection of miR-17＇ s inhibitor. Infection of Ad-miR-17 significantly suppressed the proliferation, anchorage-independent growth, in vitro invasion or migration of A549 cells. Co-transfection with miR-17 inhibitor disrupted the effect of miR-17 on A549 cells. Expression of miR-17 via identified adenoviral vector suppresses lung cancer cells proliferation.