黄芪甲苷对CCI模型所致神经损伤修复的机制初步研究

Study on the Mechanism of the Repair of Nerve Injury Induced by CCI Model by Astragaloside

大部分镇痛药在很多患者身上有镇痛效果,但是有严重的副作用,因此临床上亟需找到安全有效的能够治疗神经痛的药物。黄芪桂枝五物汤作为传统治疗药物有一定的治疗效果。黄芪甲苷为黄芪桂枝五物汤的主要成分,采用SD大鼠,用黄芪甲苷对CCI模型所致神经损伤进行修复治疗,采用免疫印迹和免疫组化等方法,研究细胞损伤修复进展情况。结果表明,AG能显著下调DRGs中P2X3、TRPA1、TRPV1表达,通过抑制这些在疼痛传递过程中有重要蛋白的表达,从而起到镇痛的作用。

Most of the pain is in the labor of many patients, but it has serious side effects. Therefore, it is ur- gent to find a safe and effective drug for treatment of neuropathic pain. Huangqi Guizhi Decoction of five ingredients as traditional drugs have a certain therapeutic effect. Astragaloside as the main component of Huangqi Guizhi De- coction of five ingredients. SD rats were used to repair the nerve injury caused by CCI model. The progress of cell damage repair was studied by using the methods of Western blot and immunohistochemistry. AG could significantly down regulate the expression of P2X3, TRPAl and TRPV1 in DRGs, which played an important role in the process of pain delivery.