红腺忍冬叶水提物抗氧化作用研究

Study on the Antioxidation of Water Extract from Leaves of Lonicera Hypoglauca Miq.

目的研究红腺忍冬叶水提物的抗氧化作用,为红腺忍冬叶的开发利用奠定科学依据。方法对红腺忍冬叶水提醇沉法分离所得各部分的样品进行体内、体外抗氧化研究。以Vc、二丁基羟基甲苯（BHT）为对照品,通过DPPH法和铁离子还原法考察红腺忍冬叶不同样品的体外抗氧化活性。以Vc为阳性对照,由D-半乳糖（1 g·kg？1）致衰的小鼠为动物模型,通过测定血清、肝脏组织中SOD、GSH-PX活性和丙二醛（MDA）含量,考察样品的体内抗氧化活性。结果红腺忍冬叶水提醇沉分离所得各部分体外抗氧化活性,在0.1-5 mg·m L-1内,90%乙醇直接沉淀物、30%乙醇沉淀物以及滤液部分具有较好的抑制DPPH自由基的活性和铁还原能力。体内SOD活性影响实验表明,30%乙醇沉淀物高剂量组、90%乙醇直接沉淀物低剂量组对提高小鼠体内SOD活性均有良好的效果,且都略优于Vc,但无显著性差异。体内GSH-PX活性影响实验表明,30%乙醇沉淀物高剂量组、90%乙醇直接沉淀物高剂量组和90%乙醇直接滤液低剂量组为佳。体内MDA含量影响实验表明,30%乙醇沉淀物高、低剂量组和30%乙醇滤液高剂量组以及90%乙醇直接沉淀物低剂量组降低MDA含量的作用最为显著。结论红腺忍冬叶水提液进一步醇沉分离所得各部分都具有不同程度的抗氧化效果,综合考虑以30%乙醇沉淀物活性最佳,值得进一步研究。

OBJECTIVE To investigate the antioxidant effects of water extract from leaves of Lonicera hypoglauca Miq. and provide a scientific basis for the development and utilization of the leaves. METHODS Study the antioxidant activities of the different ethanol sediments from water extract by the experiments in vivo and in vitro. Compared with Vc and BHT, the in vitro antioxidant activities were studied by DPPH radical scavenging and the reducing capacity of Fe3＋. Senile mouse models induced by D-galactose were used. Compared with Vc, the in vivo antioxidant activities of sediments were studied by measuring SOD, GSH-PX activity and MDA content of serum and liver tissue. RESULTS Experiments in vitro（0.1-5 mg·m L？1）： DPPH radical scavenging experiments： the 90% and the 30% ethanol precipitation and the filtrates were better than the others. The reducing capacity of Fe3＋： the 90% and the 30% ethanol precipitation and the filtrates had better effect than the other various precipitations. Experiments in vivo, the comprehensive results of SOD： the 30% precipitation（high dose group） and the 90% precipitation（low dose group） had the more excellent performance than Vc. Comprehensive GSH-PX activity results： the 30% precipitation（high dose group）, the 90% precipitation（high dose group） and the 90% filtrate（low dose group） had good activities than the other groups. Comprehensive MDA activity results： 30% precipitation（both the high and low dose group）, 30% filtrate（high dose group） and 90% precipitation（low dose group） were better than the others. CONCLUSION The various eparation components all have good antioxidant activities both in vitro and in vivo. Considering the indicators both in vitro and in vivo, the 30% achol precipitation is the best and it＇s antioxidant activity is worthy of further research.