沙参麦冬汤中3种有效成分血药浓度的UPLC-MS/MS测定及药动学研究

Determination of Plasma Consentration of 3 Active Components in Shashen Maidong Decoction by UPLC-MS/MS and Their Pharmacokinetic Study

目的建立大鼠血浆中甘草苷、花椒毒酚和甲基麦冬黄烷酮A的UPLC-MS/MS测定方法,并探讨大鼠灌胃沙参麦冬汤后其在大鼠体内的药动学过程。方法以流动相乙腈-0.1%甲酸水溶液,梯度洗脱,流速0.3 m L·min-1;采用ESI源,正负离子同时检测模式扫描,多反应监测模式（MRM）检测各成分血药浓度,并用DAS 3.0软件计算药动学参数。结果甘草苷、花椒毒酚和麦冬黄烷酮A分别在4.92-315.00 ng·m L-1、1.44-92.00 ng·m L-1和0.35-22.00 ng·m L-1线性关系良好,平均回收率均〉76.5%,日内、日间RSD均〈15%。大鼠灌胃沙参麦冬汤提取物后,甘草苷、花椒毒酚和甲基麦冬黄烷酮A的AUC0-t分别为（718.23±185.55）ng·h·m L-1,（22.52±7.53）ng·h·m L-1和（13.55±6.03）ng·h·m L-1;t1/2分别为（3.61±2.01）h,（6.93±7.78）h和（3.51±1.92）h。结论本法方便、快捷,可用于甘草苷、花椒毒素和甲基麦冬黄烷酮A的体内定量分析。

OBJECTIVE To establish an UPLC-MS/MS method for the determination of 3 components as liquiritin, xanthotoxin and methylophiopogonanone A of Shashen Maidong decoction in rat plasma, and to study the pharmacokinetics of them. METHODS The choromatography was eluted with mobile phase consisted of acetonitrile and 0.1% formic acid solution at the flow rate of 0.3 m L·min-（-1） by gradient elution. Multiple-reaction monitoring（MRM） scanning mode was employed for quantification with switching electrospray ion（ESI） source polarity in positive/negative mode. The pharmacokinetic parameters were calculated by using DAS 3.0 software. RESULTS The linear ranges of liquiritin, xanthotoxin and methylophiopogonanone A were 4.92-315.00 ng·m L-（-1）, 1.44-92.00 ng·m L-（-1） and 0.35-22.00 ng·m L-（-1）, respectively. RSDs of inta-day and inter-day were all lower than 15%, and the average recoveries were more than 76.5%. The pharmacokinetic parameters of liquiritin, xanthotoxin and methylophiopogonanone A were as follows： AUC0-t were（718.23±185.55） ng·h·m L-（-1）,（22.52±7.53） ng·h·m L-（-1）, and（13.55±6.03） ng·h·m L-（-1）; t1/2 were（3.61±2.01）h,（6.93±7.78）h and（3.51±1.92）h. CONCLUSION The method is convenient, sensitive and specific, and can be used for analysis of liquiritin, xanthotoxin and methylophiopogonanone A in vivo.