辣椒素受体基因多态性与肝胃不和型难治性反流性食管炎的相关性研究

Correlation of Transient Receptor Potential Vanilloid-1 Gene Polymorphism with Liver-Stomach Disharmony Type Refractory Reflux Esophagitis

【目的】从辣椒素受体基因(TRPV1)多态性角度阐明肝胃不和型难治性反流性食管炎(RRE)的易感因素。【方法】收集肝胃不和型RRE患者49例,健康受试者38例,采用PCR-RFLP及基因测序法检测2组受试者外周血淋巴细胞TRPV1RS8065080、RS222747、RS222749位点基因型、等位基因的分布差异。【结果】(1)RRE患者TRPV1基因位点RS8065080基因型、等位基因与健康受试者比较,差异有统计学意义(P<0.05);在显性模型下,携带CT+TT的个体患RRE的风险是携带CC个体的5.306倍(P<0.05);在共显性模型下,携带TT的个体患RRE的风险是携带CC个体的7.318倍(P<0.05),但携带CT的个体与携带CC个体比较,无统计学差异(P>0.05);在隐性模型和超显性模型下,未发现RS8065080与患RRE有关联(P>0.05)。(2)RRE患者TRPV1基因位点RS222749基因型、等位基因与健康受试者比较,差异有统计学意义(P<0.05);在共显性模型下,携带TT的个体患RRE的风险是携带CC个体的6.000倍(P<0.05),但携带CT的个体与携带CC个体比较,差异无统计学意义(P>0.05);在显性模型、隐性模型和超显性模型下,未发现RS222749与患RRE有关联(P>0.05)。(3)TRPV1基因位点RS222747基因型、等位基因与健康受试者比较,差异无统计学意义(P>0.05);在各遗传模型中,均未发现RS222747与患RRE有关联(P>0.05)。【结论】TRPV1基因RS8065080、RS222749多态性可能与肝胃不和型RRE易感性有关联,TRPV1基因RS222747多态性可能与肝胃不和型RRE易感性无关联。

Objective To clarify clinical susceptible factors of liver stomach disharmony type refractory reflux esophagitis（RRE） based on transient receptor potential vanilloid-1 （TRPV1） gene polymorphism. Methods Forty- nine cases of liver-stomach disharmony type RRE and 38 cases of healthy people were enrolled into the study. Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and gene sequencing were used to detect the distribution difference of allele and genotype of TRPV1 RS8065080, RS222747 and RS222749 in the peripheral blood lymphocytes of the two groups of subjects. Results （1） The difference of allele and genotype of TRPV1 RS8065080 in RRE patients and healthy volunteers was statistically significant（P 〈 0.05）. In dominant model, individuals carrying CT＋TT had 5.306 times higher risk of suffering from RRE than individuals carrying CC（P 〈 0.05）. In co-dominant model, individuals carrying TT had 7.318 times higher risk of suffering from RRE than individuals carrying CC （P 〈 0.05）. But there was no statistical difference between individuals carrying CT and individuals carrying CC （P 〉 0.05）. In recessive model and over-dominant model, the correlation of RS8065080 with suffering from RRE was not found （P 〉 0.05）. （2） The difference of allele and genotype of TRPV1 RS222749 in RRE patients and healthy volunteers was statistically significant（ P 〈0.05）. In the co-dominant model, individuals carrying TY had 6.000 times higher risk of suffering from RRE than individuals carrying CC （P 〈 0.05）. But there was no statistical difference between individuals carrying CT and individuals carrying CC （P 〉 0.05）. In dominant model, recessive model and over-dominant model, the correlation of RS222749 with suffering from RRE was not found（P 〉 0.05）. （3）The difference of allele and genotype of TRPV1 RS222747 in RRE patients and healthy volunteers has no statistical significance. In each model, the correlation of RS222747 with suffering from RRE was not found （P 〉 0.05）. Conclusion TRPV1 （RS8065080 and RS222749）gene polymorphism is associated with the susceptibility to liver-stomach disharmony RRE. But TRPV1 RS222747 gene polymorphism is uncertainly correlated with the susceptibility to liver-stomach disharmony RRE.